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Issue of Virologica Sinica
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Research Article
Research Article
To develop national reference of HIV-1 P24 antigen and establish the corresponding standard, specimens collected from normal population, HIV cultivated cell lysates and patients infected with HIV or suspected infection, were detected for HIV antibody, HIV P24 antigen, HCV antibody and HBsAg. 21 ..., Abstract
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Research Article
The adenovirus subgenus C serotypes 5 (Ad5) vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene ..., Abstract
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Research Article
Respiratory syncytial virus(RSV) was isolated from nasopharyngeal aspirates(NPA) collected of children suffering acute lower respiratory tract infection in the Children’s hospital of Zhejiang province in the winter of 2004. The isolated strains were identified by RT-PCR. The whole region of the G ..., Abstract
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Research Article
A simple rapid detection of HIV-1 and HIV-2 antibodies was developed by using dot immunogold filtration assay. Five recombinant proteins derived from HIV env and gag regions of HIV-1 and HIV-2 (P24, GP41, GP120C, GP120V3 and GP36) were expressed in E. coli. The proteins were purified, immobilized ..., Abstract
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Research Article
Norovirus is currently the predominant human calicivirus that causes diarrhea in China. Among the documented noroviruses, types 4, 1 and 3 are the leading genetics groups. In order to improve the expression of the norovirus capsid, we re-designed and artificially synthesized the full-length ..., Abstract
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Research Article
HIV-protease coding sequence was amplified from HIV-1IIIB RNA by one- step RT-PCR. The PCR product was inserted into the pet28a expression vector with an additional ATG start codon before and TAA stop codon after the coding sequence. Positive clone was selected and transformed into E. coli BL21 ..., Abstract
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Research Article
The glycoprotein gene(G) of 12 Rabies virus isolates from different areas in Guangxi were analyzed and sequenced. The results indicated that all rabies virus isolates from Guangxi belong to genotype I and could be divided into 3 groups. GX01, GX08, GX09, GX014, GX091, GX195, GX260 and GXLA ..., Abstract
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Research Article
In order to understand the genetic diversity and distribution of Hantavirus (HV) in different tissues of wild brown rats, we amplified the partial M segment of HV from various organs of 20 hantavirus positive Rattus norvegicus individuals which were collected from Beijing. Then the amplicons were ..., Abstract
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Research Article
In order to find the cause of different transactivate abilities among JDV, BIV and HIV-1 Tat, four chimeric Tat cDNA expression constructs, JH, HJ, JB and BJ, were generated with the crossover points at the boundary of activation and binding domains based on functional domain division of HIV-1 Tat ..., Abstract
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Research Article
In this study, the open reading frame of PPV vp2 was amplified by PCR, sequenced, cloned and expressed both in prokaryotic and insect cells. In E. coli, by using pET-32(a) expression vector, VP2 was expressed as a Trx-His-S Tag fusion protein with the size of 84 kDa and the protein was used to ..., Abstract
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Research Article
To investigate the characteritics and the mechanism of bluetongue virus strain HbC3 (BTV-HbC3) infecting MA782 malignancy in vitro,BTV-HbC3 was used to infect MA782 cell, and the cytopathic effects(CPE)was observed.Transmission electron microscope(TEM)was introduced to study changes of ..., Abstract
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Research Article
To understand the interaction between Marek’s disease viruses (MDV) and Reticuloendothe
-liosis viruses (REV) in co-infected chickens, their mutual influences on vriremia levels and specific antibody titiers were studied in REV maternal antibody positive (REV-ab+) or negative (REV-ab-) ..., Abstract
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Research Article
Seventy serum samples were collected from adult brown ducks in Hubei were collected in order to clarify the natural Duck hepatitis B virus (DHBV) infection in and to characterize the genome structure of DHBV. The complete genome of the DHBV strain was amplified by polymerase chain reaction (PCR) ..., Abstract
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Research Article
Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing ..., Abstract
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Research Article
A severe contagious disease broke out in several duckeries in Ningbo, Zhejiang province with the symptoms of diarrhea and grasping. The liver tissues from the dead ducks were collected and inoculated into the allantoic cavity of the 11-day-old embryos. Through purification by sucrose density ..., Abstract
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Research Article
A pair of primers was designed according to the sequence of vp28 gene of White spot syndrome virus (WSSV) in the GenBank. The vp28 DNA fragment was amplified by PCR and cloned into E. coli expression vector pET- 22b(+) successfully. Then pET22b-vp28 was transformed into E. coli. After IPTG ..., Abstract
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Research Article
The phospholipase A2 functional domain of PfDNV capsid gene VP1 was obtained by
RT-PCR amplification. The amplified fragment was ligated into a pMD18-T vector and sub-cloned into
prokaryotic expression vector pET28a and pET26b. The recombinant plasmid pET28a-PLA and
pET26b-PLA were used ..., Abstract
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Review
To understand the dual infection by Hepatitis B virus (HBV) and Hepatitis C virus (HCV), and the viral interference between HBV and HCV infections, biochemical and virological markers of 196 patients were detected. Among 196 patients tested, 76 patients were only infected by HBV, 72 patients were ..., Abstract
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