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Issue of Virologica Sinica
Article List:
Research Article
In order to characterize the specificity of the monoclonal antibodies (McAbs) against SARS- associated coronavirus (SARS-CoV) nucleocapsid protein and to identify the epitopes recognized by the McAbs,the nucleocapsid proteins of human coronavirus OC43 (HCoV-OC43) and 229E (HCoV-229E) was expressed ..., Abstract
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Research Article
The n and p genes from subgroup A human respiratory syncytial virus (HRSV), were amplified by RT-PCR. The n product was cloned into pGEM-T easy vector and that of p into pcDNAII vector and the two genes were subcloned into the eukaryotic expression vector pcDNA3.1(+). The resulting recombinant ..., Abstract
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Research Article
The full-length and truncated ns2 gene (2881-3078 bp) were gained through PCR using p90-HCV as template. The truncated ns2 gene was cloned into the prokaryotic expression vector PET32a. The fusion protein Trx-His-NS2C expressed in a soluble form in E.coli, and then was purified by His resin. Highly ..., Abstract
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Research Article
As one of the viral proteins expressed in the immediate-early phase of HSVI infection, ICP22 functions in the regulation of viral replication. However, it is difficult to produce specific antibody against ICP22 protein with homology of antigenicity in this protein. Based upon prediction of ..., Abstract
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Research Article
As one of the viral proteins expressed in the immediate-early phase of HSVI infection, ICP22 functions in the regulation of viral replication. However, it is difficult to produce specific antibody against ICP22 protein with homology of antigenicity in this protein. Based upon prediction of ..., Abstract
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Research Article
In this study, we constructed recombinant adenovirus containing protective antigen gene of Bacillus anthracis. The PA gene was obtained from pcDNA3.1-PA vector by PCR , and cloned into plasmid pAdTrack-CMV. The positive clone was linearized with PmeI restriction enzyme and transformed into E. coli ..., Abstract
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Research Article
The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by ..., Abstract
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Research Article
Small interference RNA mixtures (siRNA Cocktails ) of Rabies virus (RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA (dsRNA) with RNase Ⅲ. These siRNA cocktails were transfecteing into BSR or MNA cell monolayers and the direct immunofluores- cence assay (FA) was ..., Abstract
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Research Article
Equine infectious anemia virus full-length-gene chimeric clone derived virus, vLGFD9-12, was inoculated into healthy horses. Over an observation period of 150 days, all inoculated groups showed no overt abnormal symptoms. The results from routine blood assays showed that the counts of WBC and HGB ..., Abstract
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Research Article
The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting full-length cDNA of CSFV Shimen strain E2 gene into pBABE-puro. Both the recombinant retroviral vector and pVSVg plasmid were transfected into eukaryotic cells 293GP by calcium phosphate transfection method, and the ..., Abstract
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Research Article
Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6 ..., Abstract
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Research Article
An expression cassette containing the VP2 gene of infectious bursal disease virus (IBDV) strain JS under the control of the CMV promoter and an enhancer in pcDNA3.1-VP2 was amplified by PCR and cloned into pUC18-US10 plasmid to yield the transferring vector pUC18-US10-VP2. The recombinant virus ..., Abstract
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Research Article
Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire ..., Abstract
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Research Article
Eight overlapping clones covering the whole viral genome of TSV ZHZC3 isolate were amplified by RT-PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD-18T vector and sequenced. The result showed that the genome of ZHZC3 consisted of 10202nt excluding the ..., Abstract
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Research Article
Bioassay of PoNPV+1%TinopalLPW was conducted on second instar larvae of a laboratory colony of Parocneria orienta. The results showed that 1%TinopalLPW had a marked synergistic effect. Various doses of PoNPV along with 1%TinopalLPW were sprayed on second and third instar larvae of an overwintering ..., Abstract
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Research Article
The technique of immune capture reverse transcription real-time PCR (IC-RT-Realtime PCR) was applied for the first time to detect Tobacco ringspot virus. When detecting virus, it was shown to be advantageous in terms of specificity and sensitivity to integrate several techniques, such as specific ..., Abstract
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Research Article
CVB3 was propagated in Hep-2 cells and purified by sucrose gradient centrifugation. The purified CVB3 reacted with random peptides library displaying 9 amino acids. After 3 rounds of screening, the capability of phage peptides in the inhibition of CVB3 replication was determined. DNA was extracted ..., Abstract
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Research Article
The inhibitory effects of homoharringtonine、IFN-α1b 、hydroxycamptothecin and norfloxacin on Hepatitis B virus(HBV)replication in vitro was investigated. Different concentrations of each drug were added to the culture medium of HepG2.2.15 cell line transfected with HBV. The Cytopathic ..., Abstract
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Research Article
Respiratory syncytial virus ( RSV) is a worldwide leading viral cause of lower respiratory tract infection in children. Immunodeficient individuals are vulnerable to severe RSV infection. No ideal animal model is available at the present time. To establish an ideal murine model, RSV was dripped in ..., Abstract
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Research Article
A visual HSV-2 detection gene array was developed based on nanogold probes。The specific primer and probe of HSV-2 were designed based on the highly conservative region of HSV-2 DNA polymerase gene and the HSV-2 PCR amplicon were labeled by biotin through PCR reaction.Amino-modified probes were ..., Abstract
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Brief Reports
The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC ..., Abstract
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Brief Reports
The nucleoprotein (N) gene of the coronavirus of pigeon origin (PSH) was cloned and sequenced A pair of primers based on the nucleoprotein gene sequences of group 3 coronaviruses was designed and used to amplify the N gene of PSH The PCR product was purified and cloned into the pMD18-T vector. The ..., Abstract
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