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Issue of Virologica Sinica
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Research Article
The significance and preM sequence analysis of two different mutant strains of Japanese encephalitis virus during persistent infection was studied. Two wild strains of Japanese encephalitis viruses (JaGAr-01 and Nakayama) were used to infect a human hepatoma cell line. Persistent infection was ..., Abstract
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Research Article
The epitopes of SARS-CoV were screened from a 12-mer phage display random peptide library using anti- SARS-CoV horse polyclonal antibodies of as a target. The phage clones enriched in biopannings were sequenced. Two consensus sequences were obtained, DXXDP and TXTLL, which had close identity to the ..., Abstract
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Research Article
The structural protein of SARS-associated coronavirus (SARS-CoV) responsible for the activation of hfgl2 gene was investigated. Gene fragments encoding the nucleocapsid, the membrane, and spike proteins were amplified by RT-PCR, and they were cloned into the eukaryotic expression vector ..., Abstract
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Research Article
The biological Characteristics and partial S4 gene sequence of a new Reovirus isolate was determined. Cell sensitivity testing on four cell lines was conducted by using the new isolate and the data showed that L929 cells and LLC-MK2 cells were highly permissive. The reovirus was resistant to ether ..., Abstract
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Research Article
An eukaryotic expression vector phIE composed of pcDNA3 skeleton and the herpes simplex virus immediate early gene promoter was constructed to provide a new choice for ectopic expression. The plasmid phIE-Us1 was constructed by inserting the us1 cDNA downstream of the promoter to analyze the ..., Abstract
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Research Article
The aim of this study was to investigate the variation of the second envelope glycoprotein E2 of Hepatitis C Virus strains from the south of Jiangsu province and to learn about the relationship between HCV quasispecies and alanine aminotransferase (ALT). Identification genotypes of sera samples ..., Abstract
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Research Article
To examine whether the recombinant protein Hsp70-NP could induce the N-specific CTLs, we immunized C57/BL6 mice with purified protein Hsp70-NP, NP or Hsp70. The lymphoproliferative responses and CTL activities of spleen cells from vaccinated mice were determined. Accordingly, in order to get the ..., Abstract
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Research Article
The aim of this study was to characterize functions of the unknown open-reading frames, Orf7, Orf8 and Orf9 within the Severe acute respiratory syndrome coronavirus (SARS-CoV) genome. We cloned the cDNAs of Orf7, Orf8, Orf9 from the SARS-CoV BJ01 strain were cloned by RT-PCR and constructed into ..., Abstract
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Research Article
In this study, psiHBs was constructed through the backbone of psiRNA-vector to transcribe a small hairpin RNA(shRNA) corresponding to the messenger RNA of the HBV small surface antigen. After transfection of HepG2 cells or Balb/c mice with psiHBs and pHBV1.3, a plasmid containing 1.3-genomic-length ..., Abstract
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Research Article
The rationale of this study was to elucidate the etiological agent characteristics and pathogenic mechanisms of the Avian H5N1 influenza virus in mammals, and to offer a platform for evaluating vaccines and drugs against AIV. Male BALB/c mice (18~20g) were anesthetized by inhalation of ether and ..., Abstract
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Research Article
To study the specific subcellular localization of different regions of Pseudorabies virus (PrV) VP22, a series of mutants with the C-terminal truncations were amplified from a plasmid containing the full-length VP22 gene of the Ea strain. The resulting truncation constructs were fused to ORF ..., Abstract
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Research Article
The GP5 gene of Porcine reproductive and respiratory syndrome virus (PRRSV) S1 strain was amplified by RT-PCR, which was digested with KpnI and XhoI and cloned into pShuttle-CMV plasmid. Sequence analysis showed that GP5 gene was cloned in correctly. Then the recombinant plasmid pShuttle-CMV-GP5 ..., Abstract
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Research Article
Large felids, such as tigers, lions and leopards are often infected with Canine Distemper (CD). Blood samples from pre-immune Amur tiger, African lion and lynx were collected and the antibody against CDV was measured by neutralization tests. The results showed that their CDV antibody level were all ..., Abstract
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Research Article
One set of primers and a TaqMan probe were designed based on the nucleotide sequence within ORF2 of Porcine circovirus type 2 (PCV2). A quantitative TaqMan real-time PCR was developed for detecting PCV2 with highly specificity, reproducibility and more sensitivity than conventional PCR. Standard ..., Abstract
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Research Article
he RdRp gene of Foot-and-mouth disease virus was amplified by reverse transcription polymerase chain reaction (RT-PCR) using pfuultraTM High-Fidelity DNA polymerase, and was then cloned into the prokaryotic expression vector pET-28a (+). E.coli BL-21 cells were transformed with the recombinant ..., Abstract
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Research Article
The nucleocapsid protein gene (N) located 108 to 1679 situation of Canine distemper virus (CDV) genome and was immunogenicity protein with strong conservatism, so N gene was choosed as aim gene. A shuttle plasmid pVAXΔE3LPN containing the gene encoding the nucleocapsid protein of CDV was ..., Abstract
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Research Article
The VP1 gene of Swine vesicular disease virus (SVDV) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) yielding a product of 849bp cDNA fragment. Using T-A cloning technique, the PCR product was cloned into pMD18-T vector. The purified VP1 gene was subcloned into the ..., Abstract
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Research Article
Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and ..., Abstract
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Research Article
This study was aimed to analyze human rabies cases that occurred from 2004 to 2005 in Anlong County in Guizhou province, and to explore the possible factors causing the epidemics. Each case of rabies was investigated. Canine brains were collected, and an immunofluorescence assay was used to detect ..., Abstract
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Research Article
The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows ..., Abstract
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Brief Reports
Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng, 10-5ng to yield from ..., Abstract
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Research Article
By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay ..., Abstract
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