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Issue of Virologica Sinica
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Research Article
HIV-1 integrase P31 of RL42 strain, a B subtype isolate, was expressed in E.coli using pThioHisB vector(Invitrogen). The recombinant protein represented about 30% of total cellular proteins. and was purified by inclusion body washing and Ion Exchange Chromatography (IEX). The purity of P31 ..., Abstract
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Research Article
Approximately 8% of the population in China are carriers of HBV and about 5% have HCV infection. The underlying mechanisms of pathogenethis of the establishment of a chronic infection with HBV and HCV are not fully understood. Since there is no ideal model to explain chronic HBV and HCV infection ..., Abstract
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Research Article
Based on a previous study, a pGL3-5’UTR plasmid was constructed by substitution of the SV40 promoter with HCV 5’UTR cDNA. A set of deletion mutant plamids: pGL3-5’UTR1 comprising the full sequence of 5’UTR; pGL3-5’UTR2 containing domains II, III and IV; pGL3-5’UTR3 containing domains I, II ..., Abstract
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Research Article
The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L ..., Abstract
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Research Article
In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) ..., Abstract
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Research Article
Syncytin, a captive retroviral envelope protein, is possibly involved in the morphogenesis of the placental syncytiotrophoblast layer generated by trophoblast cell fusion. It is found that syncytin and HIV-1 envelope proteins share similar structural profiles and membrane fusion mechanisms. In this ..., Abstract
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Research Article
In order to amplify the complete genome of Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia Strain, eight pairs of primers were designed based on Genbank sequences. Eight fragments were obtained by PCR and were cloned into the pMD-18 T vectors . The recombinant plasmids were sequenced and the ..., Abstract
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Research Article
In this study, an improved real-time RT-PCR was applied to quantification of Foot-and-mouth disease virus in vivo and in vitro. The results suggested that this method had a high sensitivity with up to less than 10 copies / reaction. Furthermore, this novel method was successfully used to quantify ..., Abstract
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Research Article
In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully ..., Abstract
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Research Article
Membrane (M) protein genes of Canine coronavirus (CCoV) domestic strain DXMV, V1 and V2 were successfully amplified, sequenced and analyzed. The full length of all the M gene of DXMV, V1 and V2 strains was 792 bp, encoding 263 amino acids. The initial 17 amino acids as appeared to be a signal ..., Abstract
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Research Article
A pair of primers were designed based on the PrV ul24 gene sequence in GenBank (NC006151). The UL24 protein coding sequence was amplified by PCR from PrV RongA strain genomic DNA. The product was cloned into pEGFP-N1 vector to generate the plasmid pUL24-GFP. Restriction endonuclease digestion, DNA ..., Abstract
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Research Article
A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted ..., Abstract
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Research Article
A real-time reverse transcription-polymerase chain reaction (RRT-PCR) assay based on TaqMan-MGB probe was developed to rapidly detect avain influenza virus subtype H5. The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of ..., Abstract
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Research Article
The coding region of capsid protein gene from Chicken Anemia virus(CAV) was amplified from pPro-VP1 by PCR and cloned into pET-30a(+). E.coli BL21(DE3)were transformed by the recombinant plasmid pET30-VP1. Analysis by SDS-PAGE and Western blot showed that the target gene was expressed ..., Abstract
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Research Article
A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate ..., Abstract
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Research Article
A fluorescent quantitative PCR (FQ-PCR) method based on sequences of the conserved gE of the PRV genome was established and evaluated after selection of optimal reaction conditions. A pair of primers and a fluorescent TaqMan probe specific for gE gene were designed and used. We compared the FQ-PCR ..., Abstract
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Research Article
Four gene fragments of S1 strain of porcine reproductive and respiratory syndrome virus were amplified by RT-PCR and cloned into the pCR-XL-TOPO vector for sequencing. Authentic 5′ and 3′ terminal fragments of S1 strain were obtained by 5′ -Full RACE Core Set and 3′ -Full RACE Core Set and ..., Abstract
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Research Article
With the software BLAST from NCBI , the unique gene ORF22 and gene ORF40 of Spodoptera exigua nuclear polyhedrovirus (SeMNPV) were selected and cloned into the pMD18-T vector. The purified pMD18-T-ORF22 and pMD18-T-ORF40 were used as standard samples, genome of quantified SeMNPV were used as ..., Abstract
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Research Article
In this study, the bacterium strains from Takramagan in Xinjiang, which was characteristic with high temperature and strong ultraviolet (UV) radiation in summer, were isolated, purified and identified. The acetone extracts of 39 isolated strains were measured by HPLC and UV-visible detection and ..., Abstract
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Brief Reports
A new tospovirus was discovered in infected peanut plants based on complete sequencing and analysis of its genomic S RNA. It was characterized as a new strain of Capsicum chlorosis virus (CaCV) and was named China peanut (CP) strain. The total sequence of S RNA of CaCV-CP was 3399 nucleotide (nt) ..., Abstract
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Brief Reports
The association between transcriptional activation of HERV and the develop- ment of schizophrenia was investigated. Nested RT-PCR was used to detect the preval- ence of HERV env RNA in the PBMCs of individuals with recent-onset of schizophrenia and normal persons from central and southern China. ..., Abstract
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Brief Reports
Hepatitis A virus and ECHO20 viruses were isolated from faeces of hepatitis A patients in Luxi County, Yunnan Province. The combined and single serological neutralization antibody tests of enterovirus demonstrated that the patients were infected by both viruses. One strain, named LX0901, was ..., Abstract
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Brief Reports
The coat protein gene and its 3′ noncoding region of a watermelon isolate of Cucumber green mottle mosaic virus (CGMMV-LN) in Liaoning, China was determined and compared with other isolates. The gene encoding the coat protein of CGMMV-LN comprises of 486 nucleotides and encodes a putative protein ..., Abstract
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