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Research Article
The HIV-1 accessory proteins ( Nef, Vpu,Vpr and Vif ) are essential for viral replication, and may be processed for recognition by CTL. However, only limited data are available to evaluate the CTL responses against these proteins in naturally infected individuals. In this study, CTL responses ..., Abstract
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Research Article
HBV X gene was amplified by PCR and cloned into the prokaryotic expressing vector pET-his and the eukaryotic expressing vector pcDNA3.1(-). E.coli BL21 (DE3) were transformed by the recombinant plasmid pET-his-HBx.. HBx protein was expressed by IPTG induction and formed insoluble inclusion bodies ..., Abstract
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Research Article
The gene sequences of the Hemagglutinin and Nucleoprotein were downloaded from GenBank. Bioedit7.0 softwere was used to pairwise these sequences and to construct a phylogenetic tree to analyze the variation rate of measles virus strains. The results demonstrated that variation rate of hemagglutinin ..., Abstract
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Research Article
The binding of HSVⅠ to receptors of human fibroblast initiates viral infection and induces the cells to express specific proteins. SR-15, one of these proteins, was identified to interact with certain zinc finger proteins containing KRAB domain through binding zinc finger domain in yeast trap ..., Abstract
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Research Article
The polymerase gene, a non-structural gene from strain TS of transmissible gastroenteritis virus (TGEV), was amplified by RT-PCR primers designed based on the Purdue nucleotide sequence in GenBank. The expected 20054 bp product was obtained. The nucleotide sequence of ORF1 of TS shared nucleotide ..., Abstract
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Research Article
When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that ..., Abstract
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Research Article
Abstract:Using a pair of specific primers designed according to the relevant nucleotide sequence from GenBank (Accession number:DQ023145), the HA gene of AIV H5N1 subtype with the signal peptide sequence was obtained by PCR from the recombinant plasmid pUC-HA, which contains the full length of HA ..., Abstract
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Research Article
Abstract:EP0, an early protein gene of pseudorabies virus (PRV), plays important roles in viral replication and possibly in PRV latency. In order to select an siRNA that specifically inhibits the expression of EP0, three siRNA templates, EP04, EP08 and EP12, were designed and synthesized according ..., Abstract
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Research Article
The fusion protein gene of strain F48E8 of Newcastle disease virus was amplified with RT-PCR, and cloned into the pGEM-T vector and named pGEM-NDF. Then, F gene was released from pGEM-NDF by digestion with BamHI and XbaI, and ligated to baculovirus transfer vector, pFastBac1, which was digested ..., Abstract
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Research Article
Nucleocapsid (N) protein genes of 13 Infectious bronchitis virus (IBV) strains isolated from 9 provinces in China between 2000 and 2004 were sequenced and compared to 42 IBV reference strains. The results showed that all of the N genes of the 13 isolates were composed of 1230 nucleotides, but point ..., Abstract
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Research Article
An 864bp fragment at the 3’- end of the vp3 gene of Goose parvovirus (GPV) HG5/82 isolate was cloned by RT-PCR, and was cloned into pMD18-T Simple vector. Positive clones were identified by REN digestion and PCR and a recombinant plasmid was digested with BamHⅠand Hind Ⅲ and subcloned into the ..., Abstract
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Research Article
The vp19 gene of White spot syndrome virus (WSSV) was amplified by PCR with specific primers and subcloned into the prokaryotic expression vector pET32b to get the recombinant plasmid pET32b-vp19. A positive plasmid was transformed into the host cell Origami(DE3)pLysS and the target gene was ..., Abstract
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Research Article
The peptide gene of Cecropin D was amplified by RT-PCR, cloned into the expression vector pET32a to generate the recombinant plasmid pET32a-CD. E.coli BL21 (DE3) were transformed by the plasmid and it was observed that the target gene was expressed at high-level in the form of fusion protein when ..., Abstract
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Research Article
p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial ..., Abstract
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Research Article
The objective of our present study is to explore the potential use of pRNA as a bio-carrier of siRNA to inhibit HBV gene expression and replication. After co-transfected with pHA-HBs into 293T cells, HBVsi18-42, a pRNA-escorted siRNA, suppressed HBsAg and accumulated in the cells in a ..., Abstract
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Research Article
In order to determine the effect of silencing Dicer on shRNA-mediated RNA interference, we constructed two shRNA expression vectors that targeted the RNAse III domain of the Dicer gene. The shRNA vectors were transfected into 2215 cells, colon cancer TC cells and Green Fluorescent Protein ..., Abstract
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Research Article
The polypeptide fragment PrP106-126 of the cellular prion protein was utilized to construct the model of cultured cortical neurons and cerebellar granule cells. The model was used to study the viability of neurons and PrP gene expression. The results showed that the viability of cortical neurons ..., Abstract
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Research Article
A phage display random decapeptide library with high titer was constructed using pCANTAB 5 E as vector and used to select and identify antigen epitopes. ScFv A1, which specifically binds the shrimp’s White spot syndrome virus (WSSV), was used to pan against the decapeptide library and a random ..., Abstract
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Brief Reports
The gene fragment coding for amino acids 281 to 395 of the E protein of DENV-2 (New Guinea C strain) was amplified by PCR, comprising Domain III (amino acids 295 to 395) of the E protein. The fragment was cloned into pMD18-T vector and subcloned to expression vector pET-28a and pMAL-c2X. The ..., Abstract
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