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Issue of Virologica Sinica
Article List:
Research Article
SARS-CoV is a newly discovery pathogen causing severe acute respiratory syndrome, S protein play an important rule in the adsorption and penetration of SARS-CoV into the cell by interaction with ACE2 receptor. To determinant the functional motif of S protein involved in the interaction with ACE2 ..., Abstract
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Research Article
To establish a replication cellular model of hepatitis B virus (HBV) and determine its application in antiviral drug evaluation, we constructed an expression plasmid which contained 1.3 copies of the HBV genome, and measured the level of viral replication after transient transfection in Huh7 cells. ..., Abstract
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Research Article
The BALB/c mice were immunized with purified White Spot Syndrome Virus (WSSV). Six monoclonal antibody were selected, by ELISA, with VP28 protein expressed in E. Coli. The in vitro neutralization experiments showed that 4 of them could inhibit the virus infection to crayfish. Western-blot revealed ..., Abstract
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Research Article
A group of 25 rabies viruses (RABVs), recovered from 24 dogs and one human case, were collected from various areas in China between 2004 and 2006. Genetic and phylogenetic analyses of the G-L intergenic region were carried out in 25 street RABV isolates and CTN vaccine strains of 7 generations. The ..., Abstract
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Research Article
Based on the complete genome sequence of Newcastle disease virus (NDV) ZJI strain, seven pairs of primers were designed to amplify a cDNA fragment for constructing the plasmid pNDV/ZJI, which contained the full-length cDNA of the NDV ZJI strain. The pNDV/ZJI, with three helper ..., Abstract
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Research Article
The genome of a new SV40 strain(SV-IMB)isolated from a rhesus monkey was completely sequenced and compared with other isolates. The results showed that the whole genome contains 5246bp, and the average identity of SV-IMB was 98.1% as compared to other SV40 ..., Abstract
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Research Article
The M and NP gene of H5N1 avian influenza virus (A/chicken/Hubei/489/2004) were amplified by RT-PCR from viral RNA, and cloned into pMD18-T vector respectively. Then the expression plasmid containing M gene (pHM6-m) or NP gene (pHM6-np) was constructed by inserting M or NP gene into the pHM6 ..., Abstract
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Research Article
Pathogenicity of the Diatraea Saccharalis densovirus (DsDNV) was tested on its host larvae. The results showed that up to 4 days after inoculation, no larvae mortality was observed and the infected larvae started to exhibit the infection symptoms from the forth day. After 5 days of infection, the ..., Abstract
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Research Article
We developed a sensitive and rapid lateral-flow immunoassay (LFIA) for WSSV, using a colloidal gold as an indicator. The fusion protein, VP (19+28), was expressed in E. coli, purified and used to prepare polyclonal antibodies. The purified anti-VP (19+28) IgG were conjugated with colloidal gold. ..., Abstract
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Research Article
Human herpes simplex virus 1 (HSV-1) causes facial, ocular, and encephalitic disease and is associated with latent infection and cancer. Here, we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein ..., Abstract
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Research Article
A study was performed in order to validate FMDV 3ABC-I-ELISA kit developed in China for the differentiation of FMDV-infected animals from those had merely been vaccinated. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against ..., Abstract
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