Virologica Sinica, 29 (6) : 372-380, 2014
Research Article
Nucleoprotein-based indirect enzyme-linked immunosorbent assay (indirect ELISA) for detecting antibodies specifi c to Ebola virus and Marbug virus
1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

2. University of the Chinese Academy of Sciences, Beijing 100039, China
 Correspondence: yzm@wh.iov.cn
(6662.70KB)  
Abstract
Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specifi c to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in anti- Ebola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G (IgG) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA's ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specifi city. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection.
Received: 15 Dec 2014  Accepted: 30 Sep 2014  Published online: 1 Dec 2014
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