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Virologica Sinica, 30 (5) : 363-370, 2015
Research Article
Rotavirus VP7 epitope chimeric proteins elicit cross-immunoreactivity in guinea pigs
1. Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of
Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118,
China
2. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry
of Education, Yunnan Minzu University, Kunming 650500, China
 Correspondence: chenyd@imbcams.com.cn
(1340.24KB)  
Abstract
VP7 of group A rotavirus (RVA) contains major neutralizing epitopes. Using the antigenic protein VP6 as the vector, chimeric proteins carrying foreign epitopes have been shown to possess good immunoreactivity and immunogenicity. In the present study, using modified VP6 as the vector, three chimeric proteins carrying epitopes derived from VP7 of RVA were constructed. The results showed that the chimeric proteins reacted with anti-VP6 and with SA11 and Wa virus strains. Antibodies from guinea pigs inoculated with the chimeric proteins recognized VP6 and VP7 of RVA and protected mammalian cells from SA11 and Wa infection in vitro. The neutralizing activities of the antibodies against the chimeric proteins were significantly higher than those against the vector protein VP6F. Thus, development of chimeric vaccines carrying VP7 epitopes using VP6 as a vector could be a promising alternative to enhance immunization against RVAs.
Received: 30 Jun 2015  Accepted: 28 Sep 2015  Published online: 10 Oct 2015
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