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Virologica Sinica, 30 (6) : 425-432, 2015
Research Article
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii
1. Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of
Sciences, Wuhan 430071, China
2. Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical
School, Singapore 169857, Singapore
*Present address: Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an
710032, China
 Correspondence: zlshi@wh.iov.cn
Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats. Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.
Received: 16 Oct 2015  Accepted: 27 Nov 2015  Published online: 7 Dec 2015
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