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Virologica Sinica, 31 (3) : 262, 2016
Construction of an infectious cDNA clone of Tembusu virus isolated from breeder Peking ducks
College of Animal Technology and Medicine, Shandong Agricultural University, Tai’an 271018, China
 Correspondence: yxdiao@126.com
Since April 2010, an outbreak of a new disease has elicited symptoms of high fever, loss of appetite, and reduction in egg production in layer ducks in eastern China; this phenomenon has now spread throughout China (Cao et al., 2011; Su et al., 2011). The causative agent of the disease was identified as Tembusu virus (TMUV), which was classified into the genus Flavivirus, family Flaviviridae. The TMUV virion is enveloped and contains a 10, 990 nt single-stranded, positive-sense RNA genome which contains a long open reading frame. The reading frame encodes a polyprotein that is possessed into three structural (Capsid, prM and E) and seven non-structural (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) proteins by viral-encoded proteases (Tang et al., 2012; Yun et al., 2012). TMUV isolated from poultry (including chicken, duck and goose), field birds, and mosquitos, can trigger neurological symptoms and even death in mice (Liu et al., 2012; Li et al., 2013a; Liu et al., 2013; Tang et al., 2013; Tang et al., 2015). To date, the elements of TMUV required for viral replication and virulence have not been definitively determined. Reverse genetics is an effective molecular biology technique to determine the function of genomic elements responsible for the replication and virulence in positivestrand RNA viruses. Infectious cDNA clones and recombinant progeny of flaviviruses including Japanese encephalitis virus, West Nile virus, Dengue virus, Yellow fever virus, Tick-borne encephalitis virus are available. There are two major strategies to construct infectious cDNA clones of animal viruses. For example, a duck TMUV infectious cDNA clone that was transcribed into RNA via in vitro transcription has been reported (Li et al., 2013b). However, no infectious cDNA clones based on a DNA-launching strategy have been constructed and produced for progeny virus.
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Received: 21 Jun 2016  Accepted: 21 Jun 2016  Published online: 15 Jan 2016
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