Vaccine Research Center, Key Laboratory of Molecular Virology &
Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for
Biological Sciences, Chinese Academy of Sciences, Shanghai
Coxsackievirus A16 (CA16) is one of the major viral pathogens associated with hand, foot, and mouth disease. CA16 belongs to the Enterovirus genus of the Picornaviridae family and possesses a single-stranded positivesense RNA genome (Mao et al., 2014). Reverse genetics is an important tool for CA16 research. Previously, a reverse genetics T7 polymerase-based system was developed for poliovirus (Moss et al., 1989), foot-andmouth disease virus (FMDV) (Zibert et al., 1990), coxsackievirus B3 (Klump et al., 1990), enterovirus 71 (EV71) (Han et al., 2010; Shang et al., 2013), and even CA16 (Liu et al., 2011). However, in that system, cDNA must be transcribed to RNA by an exogenous T7 polymerase in vitro or in vivo. To develop a more rapid and simple method, a reverse genetics system based on human polymerase I (Pol I ) was developed for FMDV (Chang et al., 2009) and EV71 (Meng et al., 2012). In the current study, we developed a high-efficiency Pol I-driven plasmid-based reverse genetics system for CA16 (Gen- Bank: EU262658), and systemically characterized recovered CA16 particles.
Received: 15 Aug 2016 Accepted: 15 Aug 2016
Published online: 19 Apr 2016