1. Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, China
2. Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
3. Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
The relationship between the Filaggrin gene (FLG) rs2065955 polymorphism and susceptibility to Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC) was investigated in Shandong Province, China. We detected theFLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism (RFLP) in 64 EBVaGC, 82 EBVnGC, and 111 normal control samples. Immunohistochemistry was used to detect the level ofFLG protein in 35 EBVaGC and 51 EBVnGC tumor tissues. Compared with normal controls, the genotype CC and allele C ofFLG rs2065955 showed higher frequency in EBVaGC and EBVnGC. There was no significant difference between EBVaGC and EBVnGC in allele distribution ofFLG rs2065955, but the genotype CC was found more frequently in EBVaGC than in EBVnGC. The risk of developing either EBVaGC or EBVnGC in genotype CC was higher than in other genotypes. Furthermore, genotype CC ofFLG rs2065955 may contribute more to the risk of developing EBVaGC than EBVnGC. There was no significant difference in the expression level ofFLG protein between EBVaGC and EBVnGC. In conclusion, theFLG rs2065955 polymorphism was significantly related to gastric carcinoma. Allele C ofFLG rs2065955 could be a risk factor for EBVaGC or EBVnGC, while genotype CC ofFLG rs2065955 was especially associated with EBVaGC.