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Virologica Sinica, 31 (4) : 342, 2016
Detection of infectious dengue virus by selective real-time quantitative polymerase chain reaction
1. Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China
2. Second clinical medical college, Southern Medical University, Guangzhou 510280, China
3. Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
# These authors contributed equally to this work.
 Correspondence: zhangb@smu.edu.cn;zhaowei@smu.edu.cn
(875.40KB)  (923.87KB)  
The dengue virus (DENV) is a single-stranded positivesense RNA virus that belongs to the family Flaviviridae (Gubler, 2002), and has four serotypes, DENV1-DENV4, which are transmitted via Aedes aegypti and Aedes albopictus (Rodriguez-Roche and Gould, 2013). It has been reported that more than 50 million dengue infections occur each year (Guzman et al., 2010), and a serious outbreak occurred in the Southern Provinces of China in the summer of 2014. The clinical presentations of dengue infection range from mild febrile illness to severe dengue characterized by dengue hemorrhagic fever and shock syndrome, which make the accurate laboratory confirmation of the diagnosis challenging but crucial. Currently, serological assays and real-time quantitative polymerase chain reaction (qPCR) techniques are most commonly applied in the diagnosis of dengue infection (Guzman et al., 2010; B?ck and Lundkvist, 2013; Guzman and Harris, 2015). However, the current methods have some major drawbacks, such as falsepositive results owing to cross-reactivity with other arthropod- borne flaviviruses, and inability to determine the infectiousness in individual patients (Schwartz et al., 2000). Therefore, a simple and accurate method is needed to detect the virus, especially its associated infectious particles.
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Received: 15 Aug 2016  Accepted: 15 Aug 2016  Published online: 30 May 2016
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