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Virologica Sinica, 31 (5) : 444, 2016
Letter
A quantitative RT-PCR assay for rapid detection of Eurasianlineage H10 subtype influenza A virus
1. Department of Basic Sciences, College of Veterinary Medicine,
Mississippi State University, Mississippi 39762, USA
2. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
3. Animal and Plant Health Inspection Service of the US Department of Agriculture, Fort Collins 80526, USA
4. Mississippi Veterinary Research and Diagnostic Laboratory,
College of Veterinary Medicine, Mississippi State University,
Mississippi 39762, USA
 Correspondence: wan@cvm.msstate.edu
(647.76KB)  (431.37KB)  
Abstract
Quantitative RT-PCR (qRT-PCR) has been commonly used for IAV detection in influenza surveillance and disease diagnosis because of its high sensitivity, specificity, and high throughput. A number of HA subtype specific qRT-PCR methods have been developed, including H5, H7, and H9 (Monne et al., 2008). However, there is still lack of a specific qRT-PCR method for detecting the emerging H10N8 IAVs. In this study, a Eurasian-lineage H10 specific qRT-PCR is developed and validated.
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Received: 20 Jun 2016  Accepted: 20 Jun 2016  Published online: 18 Sep 2016
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