1. Stat Key Laboratory of Virology, Department of Pathogen Biology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
2. Department of Pathology and Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
3. State Key laboratory of Virology, Wuhan University, Wuhan 430072, China
In this study, we use SRLCA to identify the possible Hsp90-ORF36 interaction. Additionally, critical amino acids of ORF36 and Hsp90 are deleted or mutated to evaluate their possible contributions to Hsp90-ORF36 interaction. The results indicated that the aa 215–257 of ORF36 and aa 110–180 region of Hsp90 are essential for the Hsp90-ORF36 interaction. The M216, L221, D225, F226, W237, and T238 in ORF36 and A121, Q133, F134, T149, E158, and G168 in Hsp90 are important for the Hsp90-ORF36 interaction.
Received: 29 Dec 2016 Accepted: 29 Dec 2016
Published online: 29 Dec 2016