Virologica Sinica, 31 (6) : 509, 2016
Letter
Amino acids essential for the interaction between cellular heat shock protein 90 and a Kaposi’s sarcoma-associated herpesvirus-encoded protein kinase ORF36
1. Stat Key Laboratory of Virology, Department of Pathogen Biology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
2. Department of Pathology and Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
3. State Key laboratory of Virology, Wuhan University, Wuhan 430072, China
 Correspondence: xsun6@whu.edu.cn
(2171.53KB)  (4514.56KB)  
Abstract
In this study, we use SRLCA to identify the possible Hsp90-ORF36 interaction. Additionally, critical amino acids of ORF36 and Hsp90 are deleted or mutated to evaluate their possible contributions to Hsp90-ORF36 interaction. The results indicated that the aa 215–257 of ORF36 and aa 110–180 region of Hsp90 are essential for the Hsp90-ORF36 interaction. The M216, L221, D225, F226, W237, and T238 in ORF36 and A121, Q133, F134, T149, E158, and G168 in Hsp90 are important for the Hsp90-ORF36 interaction.
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Received: 29 Dec 2016  Accepted: 29 Dec 2016  Published online: 29 Dec 2016
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