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Virologica Sinica, 31 (6) : 521, 2016
An improved reverse genetics system for Newcastle disease virus genotype VII
1. Laboratory of Zoonosis, China Animal Health & Epidemiology Center, Qingdao 266032, China.
2. Center for Avian Diseases, Qingdao Institute of Husbandry & Veterinary Science, Qingdao 266100, China.
3. Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
 Correspondence: sunyuzhang@cahec.cn;Ding_zhuang@jlu.edu.cn
In this study, we aimed to develop a rescue system for genotype VII NDV by generation of a full-length cDNA comprising a longer ribozyme sequence. In addition to virus rescue, reporter gene expression from transfected mini-genome cDNAs was significantly enhanced by the optimized ribozyme.
Key Words:
Received: 29 Dec 2016  Accepted: 29 Dec 2016  Published online: 29 Dec 2016
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