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Virologica Sinica, 32 (3) : 199, 2017
Research Article
Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages
1. Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Shenzhen 518112, China
2. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning(CASCIRE), Chinese Academy of Sciences, Beijing 100101, China
3. China-Japan Union Hospital of Jilin University, Changchun 130033, China
4. Key Laboratory for Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China
5. Office of Director-General, Chinese Center for Disease Control and Prevention, Beijing 102206, China
6. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China
 Correspondence: beeyh@im.ac.cn;yingxialiu@hotmail.com;liulei3322@aliyun.com
The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times.There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans.The ZIKV is genetically diverse and can be separated into Asian and African lineages.A rapid,sensitive,and specific assay is needed for the detection of ZIKV across various pandemic regions.So far,the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains.To this end,we have developed a one-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay based on conserved sequences in the ZIKV envelope (E) gene.The detection limit of the assay was determined to be five RNA transcript copies and 2.94×10-3 50% tissue culture infectious doses (TCID50) of live ZIKV per reaction.The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification,when tested against other flaviviruses.The assay was also successful in testing for ZIKV in clinical samples.Our assay represents an improvement over the current methods available for the detection ZIKV and would be valuable as a diagnostic tool in various pandemic regions.
Received: 18 Feb 2017  Accepted: 18 Feb 2017  Published online: 19 May 2017
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