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Virologica Sinica, 32 (4) : 307, 2017
Research Article
Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea virus
1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China
2. Fraunhofer-Institut für Angewandte Informationstechnik FIT, Sankt Augustin 53754, Germany
 Correspondence: caoych@mail.sysu.edu.cn
(7785.45KB)  (1104.38KB)  
Abstract
Since 2010,variant strains of porcine epidemic diarrhea virus (PEDV) have caused disasters in the pork industry.The spike (S) protein,as the major immunity-eliciting antigen,has previously been used for serological testing and has been found to correlate significantly with the results of the serum neutralization (SN) test.However,further evaluation of this method is needed as new epidemic strains of PEDV emerge.Hence,the main objective of this study was to assess sow sera and determine the correlation between enzyme-linked immunosorbent assay (ELISA) results (involving a newly isolated GDS01 virus-based ELISA and ELISAs based on seven recombinant fragments comprising overlapping S1 and partial S2 sequences) and SN titers.Furthermore,we determined the reliability of the ELISAs based on receiver operating characteristics (ROC) curve analyses.For the most promising ELISA,i.e.,the SP4 ELISA,the correlation coefficient (r) and the area under curve (AUC) were determined to be 0.6113 and 0.8538,respectively.In addition,we analyzed the homology of the SP4 sequences obtained from different strains (including vaccine strains) and found that various strains showed a high degree of homology in this region.Thus,we conclude that SP4 is a promising serological testing protein for use in the field.
Received: 6 Mar 2017  Accepted: 14 Jun 2017  Published online: 20 Jul 2017
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