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Virologica Sinica, 32 (4) : 2, 2017
Letter ( Advanced Online Publication)
An improved method for identifying SUMOylation sites of viral proteins
1. CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. Wuhan Institutes of Biotechnology, Wuhan 430075, China
 Correspondence: zhengzh@wh.iov.cn
(4537.68KB)  (3663.37KB)  
Abstract
In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. Furthermore, this method provides an option for rapid and accurate identification of the potential viral protein SUMOylation sites.
Key Words:
Received: 31 Aug 2017  Accepted: 31 Aug 2017  Published online: 13 Sep 2017
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