Hello guest, Register  |  Sign In
Virologica Sinica, 33 (1) : 111, 2018
Letter
Development of Multi-analyte Suspension Assay for Simultaneously Efficient Detection of Avian Influenza Virus A Subtypes
1 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2 CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
 Correspondence: h.wang@wh.iov.cn
(521.42KB)  (593.95KB)  
Abstract
Enormous economic losses and reported cases of bird-to-human transmission alert us the importance of AIVs surveillance in natural foci. However, the limited multiplexing capacity of PCR or real-time PCR method made AIVs surveillance insufficient, costly and labor intensive. In this study, we developed a multi-analyte suspension assay (MASA) to detect AIVs subtypes based on Luminex xMAP technology. With this MASA, 7 HA and 5 NA subtypes could be identified in a single reaction. The evaluation data showed that these subtype-specific amplified primers had high specificity and the coverage of each primer pairs ranged from 82% to 97%. Our studies provide a rapid and economical method for AIVs surveillance of natural foci.
Received: 26 Dec 2017  Accepted: 31 Jan 2018  Published online: 27 Mar 2018
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved