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Virologica Sinica, 33 (2) : 201, 2018
Evaluation of Antibody-Dependent Enhancement of SARS-CoV Infection in Rhesus Macaques Immunized with an Inactivated SARS-CoV Vaccine
1 State Key Laboratory of Virology/Hubei Province Key Laboratory of Allergy and Immunology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
2 Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan 430071, China
3 Center for Animal Experiment/Animal Biosafety Level-III Laboratory, Wuhan University, Wuhan 430071, China
 Correspondence: houwei@whu.edu.cn
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It is known that vaccine-induced antibodies can aggravate the infections by the antibody-dependent enhancement (ADE) in some cases. Vaccine-induced enhancement of susceptibility to severe acute respiratory syndrome coronavirus (SARS-CoV) has also been documented. We have previously reported that an inactivated SARS-CoV Z-1 strain vaccine can effectively elicits a neutralizing and protective antibody in rhesus macaque, but the ADE occurrence still has not been considered. Thus, to ensure the safety of the inactivated SARS-CoV Z-1 strain vaccine in clinical use, the present study examined whether vaccine can trigger ADE.
The rhesus macaques were firstly immunized by the inactivated vaccine or PBS. Antibody titers were detected by 50% plaque reduction neutralization test. A model with low level neutralizing antibody was established to investigate the correlation between vaccine and disease severity. Under low level of neutralizing antibody, the clinical manifestations, pathological inspection, RT-PCR and observation of cytopathic effect were utilized to assess occurrence and consequences of infection in monkeys challenged with SARS-CoV NS-1 strain.
The clinical, virological, and pathological findings presented in this study revealed no exacerbation of infection in the rhesus macaques immunized with the inactivated SARS-CoV Z-1 vaccine following challenge with SARS-CoV NS-1. Therefore, these results preliminarily indicated that low levels of antibodies induced by the inactivated SARS-CoV Z-1 vaccine may not induce ADE in rhesus macaques, and the vaccine could be a good candidate for clinical trials.
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Received: 20 Aug 2017  Accepted: 8 Jan 2018  Published online: 14 Mar 2018
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