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Virologica Sinica, 33 (4) : 359, 2018
Research Article
Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus
1. State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, Ontario P6A 2E5, Canada
 Correspondence: wangml@wh.iov.cn
(2790.17KB)  (283.53KB)  
Abstract
The Cyclophragma undans nucleopolyhedrovirus (CyunNPV), a potential pest control agent, was isolated from Cyclophragma undans (Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G+C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames (ORFs) comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contained 13 homologous repeated sequences (hrs). Phylogenic analysis grouped CyunNPV under a distinct branch within clade "a" of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pre-transmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings implied that CyunNPV is a distinct species under Group I Alphabaculovirus.
Received: 31 May 2018  Accepted: 8 Sep 2018  Published online: 28 Aug 2018
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