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Virologica Sinica, 33 (6) : 524, 2018
Research Article
Phenotypic Characterization of Porcine IFNγ-Producing Lymphocytes in Porcine Reproductive and Respiratory Syndrome Virus Vaccinated and Challenged Pigs
1 College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
2 National Research Center for Veterinary Medicine, High-Tech District, Luoyang 471003, China
3 Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA
 Correspondence: jshi@vet.k-state.edu;tiankg@263.net
(837.62KB)  (463.75KB)  
Abstract
Porcine reproductive and respiratory syndrome (PRRS) continues to be one of the most important swine diseases worldwide. Interferon-c (IFNγ)-mediated type I cell-mediated immune response plays an important role in protection from, and clearance of, PRRS virus (PRRSV). Several lymphocyte subsets including T-helper, CTLs, Th/memory cells, and γδ T lymphocytes were previously reported to produce IFNγ during PRRSV infection. However, the proportion and phenotypic characterization of these IFNγ-secreting lymphocytes have not been explored. In this study, IFNγ producted by different lymphocyte subsets was assessed by multi-color flow cytometry after vaccination with PRRSV modified live vaccine (PRRSV-MLV) and challenge with homogeneous or heterogeneous PRRSV. The results showed that T-helper cells were the major IFNγ-secreting cell population after PRRSV-MLV vaccination and PRRSV challenge. Additionally, the proportion of IFNγ producing Th/memory cells and γδ T cells increased after PRRSV challenge. This difference was accounted for an enhanced ability to produce IFNγ in Th/memory cells and an enlarged quantity of γδ T cells. The results presented here could contribute to our understanding of the roles of IFNγ in protective immunity against PRRSV infection and may be useful for assessment of cell-mediated immunity in vaccine tests.
Received: 22 Aug 2018  Accepted: 7 Nov 2018  Published online: 17 Dec 2018
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