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Virologica Sinica, 34 (1) : 78, 2019
Research Article
Oxymatrine Inhibits Bocavirus MVC Replication, Reduces Viral Gene Expression and De-creases Apoptosis Induced by Viral Infection
1 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia, China
2 School of Clinical Medicine, Hainan Medical University, Haikou, Hainan Province, China
3 Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, Ningxia, China
 Correspondence: sunraining2008@hotmail.com
(1684.33KB)  (77.35KB)  
Abstract
Oxymatrine (OMT), as the main active component of Sophoraflavescens, exhibits a variety of pharmacological properties, including anti-oxidative, anti-inflammatory, anti-tumor, and anti-viral activities, and currently is extensively employed to treat viral hepatitis; however, its effects on parvovirus infection have yet to be reported. In the present study, we investigated the effects of OMT on cell viability, Minute virus of canines (MVC) DNA replication, viral gene expression, cell cycle, and apoptosis in Walter Reed canine cells/3873D (WRD) infected with MVC. OMT, at concentrations below 4 mmol/L(no cellular toxicity), could inhibit MVC DNA replication and reduce viral gene expression at both an mRNA and protein level, which was associated with the inhibition of cell cycle S-phase arrest in early-stage MVC infection. Furthermore, OMT significantly increased cell viability, decreased MVC-infected cell apoptosis, and reduced the expression of activated caspase 3. Our results suggest that OMT has potential application in combating parvovirus infection.
Received: 12 Sep 2018  Accepted: 13 Dec 2018  Published online: 25 Feb 2019
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