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Author [周雪平] article list of Virologica Sinica
Citrus tristeza virus (CTV) is one of the most economically important citrus viruses and harms the citrus industry worldwide. To develop reliable and effective serological detection assays of CTV, the major capsid protein (CP) gene of CTV was expressed in Escherichia coli BL21 (DE3) using ..., Abstract
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The coat protein (CP) gene and 3′noncoding region of tomato mosaic virus Sl isolate (ToMV Sl) were amplified by RT PCR with specific primers designed on the basis of reported ToMV L strain (ToMV L) sequence. The cDNA fragment was cloned and sequenced,its sequence showed that the CP gene has 480 ..., Abstract
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virus isolate(To-Sl)from tomato was collected in Ha11gzhou,Zhejiang Province.To-Slcould infect 24 plant species of three families.To-Sl produced mosaic symptoms on tomato,andlocal leision on N.tabocum White Burley. Its thern1al inactivation point was 85~90℃,dilutionend point was ..., Abstract
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A virus isolate(B一935A)from faba 13e2.n w collected in Hangzhou.Zhejiang province.The isolate could infect 1 2 plant species in tkrce families. On faba bean,the isolate produced mosaic symptoms and necrosis.Its thermal inactivaflon point was 85— 90℃ ,dilution end po int was 1 0一一1 0 ..., Abstract
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CMV isolate(CMVP 1)containing satellite RNA was obtained from pea(Pisum sativum)in Jiangsu province in 1990.Twenty-nine plant species representing nine families were inoculated mechanically with CMVP l and no symptoms or only mild symptoms were found.In Nicotina tabacum-Samsum,CMVP 1 caused Slight ..., Abstract
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Comparative studies were made among five CMV isolates cullected from pea(Pisum sativum),hyacinth bean(Dolichus lablab),bean(Phaseolus vulgaris)and red bean(P.angularis),which were namedCMVP_2 CMVP_3,CMVHB,CMVB and CMVRB,respectively.No difference was found in stability insap,aphid ..., Abstract
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TuMV, SMV, and CMV were detected by dot-immunobinding assay (DIA) at dilutions of 1:5120, 1:2560 and 1:1280, respectively. DIA successfully detected 1.7ng of purified SMV and 1.2ng of purified CMV. DIA was used with horseradish peroxidase-linked goat anti-rabbit IgG and nitrocellulose membrane was ..., Abstract
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