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Author [
张源
] article list of
Virologica Sinica
An improved method for identifying SUMOylation sites of viral proteins
Yan Liu
,
Zhongyuan Tan
,
Bo Shu
,
Yuan Zhang
,
Caishang Zheng
,
Xianliang Ke
,
Xi Chen
,
Hanzhong Wang
,
Zhenhua Zheng
In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of ...,
Abstract
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2017 Vol. 32 (6)
: 537
The Humoral and Cellular(CTL)Immunological Property of HCV E2 Protein
GUO Tai-lin
,
YE Lin-bai
,
GAO Jin-rong
,
WU Zheng—hui
,
SHE Ying—long LIAO Qing-jiao
,
YANG Xiao-jun
,
CHEN Xiao—xi
,
ZHANG Yuan
The E2 gene of Hepatitis C Virus was amplified by PCR.and cloned into the pQE30 at the
downstream of LacZ promoter.The recombinan t vector DNA was introduced into the E.coli盯 109 cell
to create the JM109 pQE2.The HCV E2 protein fused to 6 His peptides with molecular weight of
35kDa ...,
Abstract
Free PDF
(364.79KB)
2003 Vol. 18 (3)
: 206-212
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