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Nonenveloped animal viruses must disrupt the host cell membrane to initiate infection. Although myristoylated outer capsid protein is known to be responsible for membrane penetration in nonenveloped viruses, the mechanisms underlying membrane penetration to enter the host cytoplasm remain poorly ..., Abstract
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In this study, we characterized a QD-labeled nonenveloped aquareovirus. Our results showed that QDGCRVs maintained their native particle properties and showed excellent infectivity in host cells. To the best of our knowledge, this is the first study reporting the realization of in situ real-time ..., Abstract
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Aquareovirus species vary with respect to pathogenicity, and the nonstructural protein NS80 of aquareoviruses has been implicated in the regulation of viral replication and assembly, which can form viral inclusion bodies (VIBs) and recruit viral proteins to its VIBs in infected cells. NS80 ..., Abstract
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Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in ..., Abstract
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Grass carp reovirus (GCRV) is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprised of five core proteins and two outer capsid proteins. The ..., Abstract

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Grass carp reovirus (GCRV), a double stranded RNA virus that infects aquatic animals, often with disastrous effects, belongs to the genus Aquareovirus and family Reoviridea. Similar to other reoviruses, genome replication of GCRV in infected cells occurs in cytoplasmic inclusion bodies, also called ..., Abstract
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Genome replication of reovirus occurs in cytoplasmic inclusion bodies called viral factories or viroplasms. The viral nonstructural protein μNS, encoded by genome segment M3, is not a component of mature virions, but is expressed to high levels in infected cells and is concentrated in the infected ..., Abstract
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Sequences analysis revealed Grass carp reovirus (GCRV) s10 was 909 nucleotides coding a 34 kDa protein denoted as VP7, which was determined to be a viral outer capsid protein (OCP). To obtain expressed OCP in vitro, a full length VP7 gene was produced by RT-PCR amplification, and the amplified ..., Abstract
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Grass carp reovirus (GCRV), a disaster agent to aquatic animals, belongs to Genus Aquareovirus of family Reoviridea. Sequence analysis revealed GCRV genome segment 8 (s8) was 1 296 bp nucleotides in length encoding an inner capsid protein VP6 of about 43kDa. To obtain in vitro non-fusion expression ..., Abstract
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In order to compare the difierence of protein expression in A utograph cdifornica nu,cleo,7
polyhedrosis virus(AcMNPV)mutants,the spodopterafrugiperda IPLB-SF-21 Cell was infected with
wild type(HR3)and mutants(ts317,ts538,ts8),and incubated at permissive and nonpermissive ..., Abstract
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Abstract:Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively
studied at the first time.It showed that the stored GCRV873,GCRVs75 and GCRv876 at minus 3012 for
ten years still remained infectious to CIK cells,and the 3 virus titers could reach above 10 ..., Abstract
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The structure and morphogensis of Mandarin fish( Siniperca chuats ) virus were studied.The matured virons were about 135nm±10 in diameter with envelope outside.The intact virus is composed of three parts,its nucleocapsid was about 90nm±5,and the envelope was around 18nm±3,the air space between core ..., Abstract
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An icosahedral virus was found in the ultra thin section of spleen, kidney, liver, gill of the outbreak infectious Siniperca chuatsi by electron microscope. The virions of section show hexagon, about 125 150 nm in diameter, and have envelope outside. Cell culture indicated that CIK cell was ..., Abstract
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The morphogenesis of the Grass Carp Reovirus (GCRV) was studied in CIK cells. The CIK cells were infected by the GCRV at MOI (multiplicity of infection) of 5 10 PFU/Cell. It was demonstrated that the subviral particles without outer layer proteins could be seen in the cytoplasm at 4 hours post ..., Abstract
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Grass Carp Hemorrhage Virus (GCHV) was purified from infected fish cell culture by using different centrifugation. Its total genome was isolated by proteinase K treatment following phenol/chloroform extraction. The individual segment of GCHV dsRNA was separated by running 1% agarose gel ..., Abstract
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By studying the flue structure of GCHV (Grass Carp Hemorrhage Virus), It was found that the capsid of purified GCHV can be degraded spontaneously into its morphological units by prologing in a buffer of low ionic strength, but not very completely. The virus preparation was obtained from GCHV ..., Abstract
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The immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric ..., Abstract
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ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation ..., Abstract
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