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Author [郭德银] article list of Virologica Sinica
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV). SFTSV is associated with a high mortality rate and has been reported in China, South Korea and Japan. SFTSV undergoes rapid changes owing to evolution, gene mutations, and reassortment ..., Abstract
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The 5′-cap structures of eukaryotic mRNAs are important for RNA stability, pre-mRNA splicing, mRNA export, and protein translation. Many viruses have evolved mechanisms for generating their own cap structures with methylation at the N7 position of the capped guanine and the ribose ..., Abstract
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Severe acute respiratory syndrome coronavirus (SARS-CoV) encodes eight accessory proteins, the functions of which are not yet fully understood. SARS-CoV protein 6 (P6) is one of the previously studied accessory proteins that have been documented to enhance viral replication and suppress host ..., Abstract
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The M and NP gene of H5N1 avian influenza virus (A/chicken/Hubei/489/2004) were amplified by RT-PCR from viral RNA, and cloned into pMD18-T vector respectively. Then the expression plasmid containing M gene (pHM6-m) or NP gene (pHM6-np) was constructed by inserting M or NP gene into the pHM6 ..., Abstract
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SARS coronavirus(SARS-CoV) is a novel human coronavirus and was responsible for SARS outbreak in several regions of the world in 2003.The genome of SARS-CoV is about 30,000 nucleotides in length and sequence analysis revealed that nucleotides 1-72 contain a RNA leader sequence preceding an ..., Abstract
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Abstract:To determine if SARS—associated coronavirus(SARS—CoV)induces apoptosis.we studied the
SARS—CoV infected Vero E6 cells by agarose gel electrophoresis.indirect fluorescent staining for
infection an d Hoechst 33528 staining for nuclei,as well as by flow cytometry.It was found ..., Abstract
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Abstract:In this study,we inserted the 5 untranslated region(UTR)of Hepatitis C virus(HCV)
genome into the upstream of the reporter genes of enhanced green fluorescent protein (eGFP)and
luciferase.an d we also constructed the expression vector that Can express the short interfering RNAs ..., Abstract
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