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Author [陈新文] article list of Virologica Sinica
Hepatitis C virus (HCV) infects approximately 180 million people worldwide. Significant progress has been made since the establishment of in vitro HCV infection models in cells. However, the replication of HCV is complex and not completely understood. Here, we found that the expression of ..., Abstract
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Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are ..., Abstract
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Abstract: Fibroblast growth factor (FGF) is a key regulator of developmental processes. A FGF homolog (vFGF) is found in all lepidopteran baculoviruses. Autographa californica nucleopolyhedrovirus (AcMNPV) and Bombyx mori NPV (BmNPV) vFGFs are chemotactic factors. Here we analyzed the vfgf of ..., Abstract
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Baculoviruses produce two viral phenotypes, the budded virus (BV) and the occlusion-derived virus (ODV). ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. ..., Abstract
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Baculovirus has many advantages as vectors for gene transfer. We demonstrated that recombinant baculovirus vectors expressing p35 (Ac-CMV-p35) and eGFP (Ac-CMV-GFP) could be transduced into human kidney 293 cells efficiently. The level of transgene expression was viral dose dependent and high-level ..., Abstract
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The full-length and truncated ns2 gene (2881-3078 bp) were gained through PCR using p90-HCV as template. The truncated ns2 gene was cloned into the prokaryotic expression vector PET32a. The fusion protein Trx-His-NS2C expressed in a soluble form in E.coli, and then was purified by His resin. Highly ..., Abstract
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In this work,the truncated Hepatitis C virus(HCV) structural protein E1 fused with GST was expressed in E.coli.The fusion protein was used as an antigen to immunize rabbit to produce the antibody for detection.To express HCV E1 in insect cells,the recombinant baculovirus(vAcHCVE1) containing the ..., Abstract
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Baculoviruses and entomopoxvirus are the viruses which have been reported so far having ubiquitin gene. The ubiquitin gene of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus(HaSNPV) was amplified by PCR. Nucleotide analysis showed that it was 252bp,encoded 83 amino acids with a ..., Abstract
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Autographa californica multicapsid nucleopolyhedrovirus(AcMNPV) blocked the Tn-Hi5 cell apoptosis stimulated by Helicoverpa armigera nucleopolyhedrovirus (HaSNPV). Futhermore AcMNPV could rescued HaSNPV replication in Tn-Hi5 cell and produced infective virus. This means that HaSNPV extend the host ..., Abstract
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Using a pair of specific primers designed according to the nucleotide sequence of Japanese encephalitis virus and Hameoaggulatinin of Human influenza virus from GenBank, the gene of JEV E protein’s main antigenic domain including the sequence of Human influenza virus ha gene was amplified with PCR ..., Abstract
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To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of ..., Abstract
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Autographa californica nucleopolyhedrovirus (AcMNPV) is the typical member of nucleopolyhedrovirus of Baculoviradae, a circled and double stranded large DNA virus. It’s also one of the most widely studied baculoviruses that turns out to be environment sound pesticide and a powerful vector of ..., Abstract
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Abstract:Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the
genome DNA of HaSNPV.The PCR products were cloned into pBluescript KS(+)plasmid.The genes
were introd uced into the expression vector pProExHTb.After IFFG induction.the E coli DH5a strain ..., Abstract
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Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera
exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As
pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO ..., Abstract
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分子伴侣(molecularchaperone)的概念,由Lasky于1978年首先提出,真核细胞内质网中的分子伴侣是由多种蛋白质组成,它 们可以介导新合成蛋白质的正确折叠与装配,并在真核生物的细胞中广泛存在。, Abstract
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The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal
peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres—
sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed ..., Abstract
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A 8.5kb fragment containing an E .coli low—copy number mini F replicon,a selectable
kanamycin resistance marker and lacZ gene with attTn7(the target site for bacterial transposon Tn7)
replaced polyhedrin gene in HaSNPV genome using homologous recombination.HaSNPV genome was
cloned ..., Abstract
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The Xba I-I fragment located at 18.4~22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which ..., Abstract
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In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a ..., Abstract
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In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8~59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular ..., Abstract
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