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Author [陈新文] article list of Virologica Sinica
Hepatitis C virus-induced prion protein expression facilitates hepatitis C virus replication
Huixia Zhang, Shanshan Gao, Rongjuan Pei, Xinwen Chen, Chaoyang Li
Hepatitis C virus (HCV) infects approximately 180 million people worldwide. Significant progress has been made since the establishment of in vitro HCV infection models in cells. However, the replication of HCV is complex and not completely understood. Here, we found that the expression of ..., Abstract
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2017 Vol. 32 (6): 503
Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived (Huh7) cells
Ming Zhou, Kaitao Zhao, Yongxuan Yao, Yifei Yuan, Rongjuan Pei, Yun Wang, Jizheng Chen, Xue Hu, Yuan Zhou, Xinwen Chen, Chunchen Wu
Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are ..., Abstract
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2017 Vol. 32 (6): 465
The open reading frame 113 of Heliocoverpa armigera single nucleopolyhedrovirus encodes a functional fibroblast growth factor
Xiang LI, Chang-yong LIANG, Jian-hua SONG, Xin-wen CHEN
Abstract: Fibroblast growth factor (FGF) is a key regulator of developmental processes. A FGF homolog (vFGF) is found in all lepidopteran baculoviruses. Autographa californica nucleopolyhedrovirus (AcMNPV) and Bombyx mori NPV (BmNPV) vFGFs are chemotactic factors. Here we analyzed the vfgf of ..., Abstract
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2008 Vol. 23 (5): 321-329
Baculovirus per os Infectivity Factors Are Involved in HearNPV ODVs Infection of HzAM1 Cells in vitro*
Ting JIANG＃, Xiang LI, Jian-hua SONG, Chang-yong LIANG, Xin-wen CHEN
Baculoviruses produce two viral phenotypes, the budded virus (BV) and the occlusion-derived virus (ODV). ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. ..., Abstract
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2008 Vol. 23 (1): 25-30
Baculovirus-mediated Expression of p35 Confers Resistance to Apoptosis in Human Embryo Kidney 293 cells*
Jian-hua SONG, Chang-yong LIANG, Xin-wen CHEN
Baculovirus has many advantages as vectors for gene transfer. We demonstrated that recombinant baculovirus vectors expressing p35 (Ac-CMV-p35) and eGFP (Ac-CMV-GFP) could be transduced into human kidney 293 cells efficiently. The level of transgene expression was viral dose dependent and high-level ..., Abstract
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2007 Vol. 22 (5): 389-396
Cloning of HCV ns2 Gene and its Expression in Prokaryotic and Mammalian cells
LIU Chao-hong, JIAO Chen-song, CHEN Xin-wen
The full-length and truncated ns2 gene (2881-3078 bp) were gained through PCR using p90-HCV as template. The truncated ns2 gene was cloned into the prokaryotic expression vector PET32a. The fusion protein Trx-His-NS2C expressed in a soluble form in E.coli, and then was purified by His resin. Highly ..., Abstract
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2006 Vol. 21 (3): 217-220
Expression and Localization of the HCV Structural Protein E1 in Insect Cells
MA Jia, WANG Rui-qing, SONG Jian-hua, LIANG Chang-yong, CHEN Xin
In this work,the truncated Hepatitis C virus(HCV) structural protein E1 fused with GST was expressed in E.coli.The fusion protein was used as an antigen to immunize rabbit to produce the antibody for detection.To express HCV E1 in insect cells,the recombinant baculovirus(vAcHCVE1) containing the ..., Abstract
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2005 Vol. 20 (5): 480-484
Cloning and Expression of Ubiquitin Gene of Helicoverpa armigera Single-nucleocapsid Nucleo- polyhedrovirus and its Antiserum Preparation
LI Jing-jing, YAO Qing, YANG Dong-ye, YU Ze-hua, CHEN Xin-wen
Baculoviruses and entomopoxvirus are the viruses which have been reported so far having ubiquitin gene. The ubiquitin gene of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus(HaSNPV) was amplified by PCR. Nucleotide analysis showed that it was 252bp,encoded 83 amino acids with a ..., Abstract
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2005 Vol. 20 (3): 298-302
AcMNPV P35 Inhibit Apoptosis Induced by HaSNPV in Tn-Hi5 Cell
NAN Fang, SONG Jian-hua, WANG Rui-qing, LIANG Chang-yong, CHEN Xin-wen
Autographa californica multicapsid nucleopolyhedrovirus(AcMNPV) blocked the Tn-Hi5 cell apoptosis stimulated by Helicoverpa armigera nucleopolyhedrovirus (HaSNPV). Futhermore AcMNPV could rescued HaSNPV replication in Tn-Hi5 cell and produced infective virus. This means that HaSNPV extend the host ..., Abstract
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2005 Vol. 20 (2): 173-178
Expression of HCV Structural Proteins and Self-assembly of HCV-like Particles in Insect Cells
WANG Rui-qing, LIANG Chang-yong, JIAO Cheng-song, SONG Jian-hua, CHEN Xin-wen
Using a pair of specific primers designed according to the nucleotide sequence of Japanese encephalitis virus and Hameoaggulatinin of Human influenza virus from GenBank, the gene of JEV E protein’s main antigenic domain including the sequence of Human influenza virus ha gene was amplified with PCR ..., Abstract
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2005 Vol. 20 (1): 5-10
Function of Actin in Transportation of the AcMNPV from Nuclear to out side of Cell
LI Xiao-qing, ZHOU Rui, JIA Yun-li, JIA Fang, YU Ze-hua, CHEN Xin-wen
To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of ..., Abstract
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2004 Vol. 19 (6): 630-635
Functional Analysis of AcMNPV P78/83 Protein
NIE Ying-chao, FANG Ming-gang, WANG Qian, YU Ze-hua, CHEN Xin-wen
Autographa californica nucleopolyhedrovirus (AcMNPV) is the typical member of nucleopolyhedrovirus of Baculoviradae, a circled and double stranded large DNA virus. It’s also one of the most widely studied baculoviruses that turns out to be environment sound pesticide and a powerful vector of ..., Abstract
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2004 Vol. 19 (5): 498-503
Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies
CHEN M ing—ruing, WU Dong, YUAN Li, CHEN Xin—wen, HU Zhi—hong
Abstract：Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the
genome DNA of HaSNPV．The PCR products were cloned into pBluescript KS(+)plasmid．The genes
were introd uced into the expression vector pProExHTb．After IFFG induction．the E coli DH5a strain ..., Abstract
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2003 Vol. 18 (6): 597-602
Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus
CHENG Rui’, LIANG Chang．yong, NAN Fang, HU Zhi．hong, VLAK M ．Just, CHEN Xin-wen
Abstract：Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera
exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides．As
pe sticides，both viruses have very narrow host range and Can not make cross infection．Trying tO ..., Abstract
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2003 Vol. 18 (6): 587-592
Molecular Chaperones and Virus Glycoprotein
WAN Qi, ZHANG Xiao-Lian ”, CHEN Xin-wen
分子伴侣(molecularchaperone)的概念，由Lasky于1978年首先提出，真核细胞内质网中的分子伴侣是由多种蛋白质组成，它 们可以介导新合成蛋白质的正确折叠与装配，并在真核生物的细胞中广泛存在。, Abstract
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2002 Vol. 17 (4): 377-380
Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli
WU Dong, W ANG Hua—lin, DENG Fei, CHEN Xin—wen, PENG Hui—yin。HU Zhi—hong
The PCR product of the HaSNPV chitinase gene，which was without the N—terminal signal
peptide and the C-terminal endoplasmic reticulum location signal，was cloned into a prokaryotic expres—
sion vector pProEXHTb．After the induction of IPTG，the chitinase gene was successfully expressed ..., Abstract
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2002 Vol. 17 (4): 331-335
A Novel Expression System of Helicoverpa armigera single-nucleocapsid nucleopolydrovirus
W ANG Han—zhong, HUANG Yi, SI Yan—hong, FANG Ming．gang ． CHEN Xinwen, Just M ．Vlak ．HU Zhi．hong
A 8．5kb fragment containing an E ．coli low—copy number mini F replicon，a selectable
kanamycin resistance marker and lacZ gene with attTn7(the target site for bacterial transposon Tn7)
replaced polyhedrin gene in HaSNPV genome using homologous recombination．HaSNPV genome was
cloned ..., Abstract
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2002 Vol. 17 (4): 319-325
Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera
FENG Zong—di, CHEN Xin—wen, Just M ．Vlak, HU Zhi—hong
The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which ..., Abstract
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2002 Vol. 17 (2): 125-131
Sequence Analysis of the H／rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)
FANG Ming-gang, CHEN Xin—Well, WANG IJuan-zhong, Just M．Ⅵak, HU Zhi-hong
In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a ..., Abstract
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2001 Vol. 16 (4): 355-360
Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene
FAN Lingyun, HU Zhihong, Just．M．Vlak, CHEN Xinwen
In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8～59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular ..., Abstract
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2001 Vol. 16 (4): 344-349
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