Hello guest, Register  |  Sign In
Author [CHEN Xin-Wen] article list of Virologica Sinica
The full-length and truncated ns2 gene (2881-3078 bp) were gained through PCR using p90-HCV as template. The truncated ns2 gene was cloned into the prokaryotic expression vector PET32a. The fusion protein Trx-His-NS2C expressed in a soluble form in E.coli, and then was purified by His resin. Highly ..., Abstract
Free PDF (440.39KB)
Baculoviruses and entomopoxvirus are the viruses which have been reported so far having ubiquitin gene. The ubiquitin gene of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus(HaSNPV) was amplified by PCR. Nucleotide analysis showed that it was 252bp,encoded 83 amino acids with a ..., Abstract
Free PDF (400.82KB)
Autographa californica multicapsid nucleopolyhedrovirus(AcMNPV) blocked the Tn-Hi5 cell apoptosis stimulated by Helicoverpa armigera nucleopolyhedrovirus (HaSNPV). Futhermore AcMNPV could rescued HaSNPV replication in Tn-Hi5 cell and produced infective virus. This means that HaSNPV extend the host ..., Abstract
Free PDF (450.43KB)
Using a pair of specific primers designed according to the nucleotide sequence of Japanese encephalitis virus and Hameoaggulatinin of Human influenza virus from GenBank, the gene of JEV E protein’s main antigenic domain including the sequence of Human influenza virus ha gene was amplified with PCR ..., Abstract
Free PDF (569.88KB)
To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of ..., Abstract
Free PDF (713.95KB)
Autographa californica nucleopolyhedrovirus (AcMNPV) is the typical member of nucleopolyhedrovirus of Baculoviradae, a circled and double stranded large DNA virus. It’s also one of the most widely studied baculoviruses that turns out to be environment sound pesticide and a powerful vector of ..., Abstract
Free PDF (1336.70KB)
Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera
exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As
pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO ..., Abstract
Free PDF (308.01KB)
分子伴侣(molecularchaperone)的概念,由Lasky于1978年首先提出,真核细胞内质网中的分子伴侣是由多种蛋白质组成,它 们可以介导新合成蛋白质的正确折叠与装配,并在真核生物的细胞中广泛存在。, Abstract
Free PDF (195.06KB)
A putative ν-cathespin(cath)gene was identified from the single(S) nucleocapsid nucleopolyhedrovirus of Helicoverpa armigera(HaSNPY).This open reading frame is 1098 nucleotides long,encoding a putative protein of 366 amino acids.The amino acids sequence alignment of eleven baculoviral v-CATHs ..., Abstract
Free PDF (279.00KB)
The development of Dendrolimus punctatus wenshanensis Cytoplasm Polyhedrosis Virus (DpwCPV) insecticide is reported. The formulation is an oil in water emulsion and the concentration of polyhedrosis is 2.5×10 9 PIB/mL. The auxiliary materials come from the agriculture products. It is safety ..., Abstract
Free PDF (181.77KB)
In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading ..., Abstract
Free PDF (291.39KB)
The polyhedrin gene of the Heliothis armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) was localized by Southern blot hybridization with a probe from the Autographa cali-fornica MNPV (AcMNPV) polyhedrin gene. Nucleotide sequencing showed the open reading frame of the HaSNPV polyhedrin gene ..., Abstract
Free PDF (259.01KB)
The paper reports paroceneria furva single nuclear polyhedrosis virus(PfNPV)that was not described before.The size of mlyhe0m inclusion bodies(Pros)raiages from 1.0um to 1.35um.The virions are rod—shaped,approximately 371× 73nm., Abstract
Free PDF (75.97KB)
A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant ..., Abstract
Free PDF (342.75KB)
We isolated a new baculovirus—Pavoenerin orient Nuclear Polyhedrosis Virus (PoNPV). The polyhedral IBs range in size from 1.06—2.42μm. The virions are rod-shoped, approximately 385×55μm. The rate of death was 95%when P.orient laraves were infected with PoNPV., Abstract
Free PDF (91.87KB)
Up1Next20/Page, 1 Pages , Go to page  
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved