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Author [CUI Zhi-zhong] article list of Virologica Sinica
An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence ..., Abstract
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To understand the interaction between Marek’s disease viruses (MDV) and Reticuloendothe
-liosis viruses (REV) in co-infected chickens, their mutual influences on vriremia levels and specific antibody titiers were studied in REV maternal antibody positive (REV-ab+) or negative (REV-ab-) ..., Abstract
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Avian reticuloendotheliosis virus (REV) infection was reported to be very common in chicken flocks in China, but its economic impact on the poultry industry was not clear. The results in this study indicated that REV infection in 1-day-old SPF chickens could severely ..., Abstract
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A very virulent strain SD0210 of Infectious bursal disease virus (vvIBDV) was adapted to chicken embryo fibroblast cell (CEF) after propagating in SPF chicken embryo for 10 generations and CEF for 18 generations. The test of pathogenicity, virulent stability and immunogenicity in chickens showed ..., Abstract
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pp38 gene and pp24 gene of MDV Md11 strain were cloned into pBudCE(4.1) vector respectively. The pBudCE(4.1)-pp38 and pBudCE(4.1)-pp24 eukaryotic expressing plasmids were transfected into CEF respectively by lipofectamine reagent. 24, 48, 72 hours after transfection, the expression of pp38 was ..., Abstract
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In this study, sequences of the 1.8kb gene family of 12 different strains of Marek′s disease vivuses were compared. These strains covered 4 different pathotypes, i.e, vaccine, virulent, very virulent, and very virulent plus strains, plus three field strains isolated in China. The results ..., Abstract
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The tissues samples of 828 chickens suspected to be infected by immunodepressive viruses from 42 shicken flocks were collected in Shandong, Henan,Hebei,Beijing,Jiangsu,Guangdong,Guangxi,Sichuan,Jilin,Liaoning and Taiwan,and the DNA was extracted for hybridization in Dot-blot with probes for ..., Abstract
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An infectious bursal disease virus (IBDV), isolated from Dongying city in Shandong province, was propagated several generations in SPF embryos and then turned to cell culture. The chickens were immunized by 20th, 21st, and 25th generation cell viruses in different doses at the age of 7 d and 14 d ..., Abstract
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pp24 gene was amplified from genomic DNA of Marek’s disease viruses (MDV) Md11 strain by polymerase chain reaction(PCR) and then cloned into pGEX-6P-1 according to the right open reading frame(ORF). The recombinant plasmid was transformed into E.coli BL21 strain for expression with the induction ..., Abstract
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The long terminal repeat (LTR) of Reticuendotheliosis virus (REV) was amplified by PCR technique and cloned into pUC18 vector at the sites of EcoRI and SacI, and the BGH polyadenylation signal sequence was cloned at the sites of Sph I and Hind Ⅲ as a terminor. The positive clone was named pUC-LTR ..., Abstract
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Four field strains of Infections bursal disease viruses (IBDV) isolated in China during 1997~1999 were compared for their pathogenity and sequences in high variable (hv) region of vp2 genes. It was indicated that GX8/99 was a very virulent IBDV strain (vvIBDV), pathogenicity of other 3 strains was ..., Abstract
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Abstract:One day-old unvaccinated chicks were inoculated with serotype I virulent strain GA and vac—
cine strain CVI988.semtype III vaccine strain HVT of Marek’s disease viruses(MDV).By using an
indirect immunofluorescence assay of suspensions of peripheral blood mononuclear ..., Abstract
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The complete encoding sequence with the initiation and stop codons ofMarek s disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I ..., Abstract
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