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Author [DENG Fei] article list of Virologica Sinica
Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency and necessity to develop high-quality influenza vaccines in a safer, more efficient and ..., Abstract
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As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These ..., Abstract
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Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV ..., Abstract
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p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial ..., Abstract
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The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes ..., Abstract
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The cDNA and genomic DNA of ribosomal protein L13 (RpL13) gene were cloned from Helicoverpa zea cells(HzAM1). Sequence analysis indicated that the open reading frame of rpL13 was composed of 666bp without intron,encoding a protein of 25 kDa. The sequence was used to construct phylogentic trees with ..., Abstract
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There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using ..., Abstract
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The PCR product of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus HaSNPV fp25k gene was cloned into a prokaryotic expression vector pProEXHTb. After inducing with IPTG, the fp25k gene was successfully expressed in E.coli DH5α. The expression product was 32 kDa. This protein was ..., Abstract
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The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The ..., Abstract
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Abstract:Here we report the pathogensis process of Helicoverpa armigera larvae infected with
Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)using H.E staining and
immunohistoc hemistry with an tibody against polyhedrin.The fourth instar Helicoverpa armigera larvae ..., Abstract
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The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal
peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres—
sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed ..., Abstract
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