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Author [DONG Jiang-Li] article list of Virologica Sinica
The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. ..., Abstract
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The intergenic sequence (IS) cDNA of Potato Leafroll Virus (PLRV) Chinese isolate was constructed into transformation binary vector pROK2 in positive or negative direction, then introduced into the commercial potato cultivar Desiree via Agrobacterium tumefaciens mediated gene transfer. Kanamycin ..., Abstract
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Two specific primers were designed and synthesized according to the genomic sequence ofPLRV reported in literature,The first strand of cDNA was synthesized by reverse-transcriptionusing RNA of PLRV Chinese isolate(PLRV-Ch)as a template,followed by PCR amplification.The synthesized cDNA was cloned ..., Abstract
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