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Author [FANG Qin] article list of Virologica Sinica
Currently, three predominant subtypes of influenza virus are prevalent in pig populations worldwide: H1N1, H3N2, and H1N2. European avian-like H1N1 viruses, which were initially detected in European pig populations in 1979, have been circulating in pigs in eastern China since 2007. In this study ..., Abstract
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Grass carp reovirus (GCRV) is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprised of five core proteins and two outer capsid proteins. The ..., Abstract

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Aquareoviruses,which belong to a newly identified genus of the family Reoviridae,are
agents infecting all kind of aquatic animals.Grass carp reovirus(GCRV)was isolated from outbreak
grass carp fingerling in South China freshwater region,and Threadfin reovirus(1f1W )is a Singapore ..., Abstract
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Abstract:Grass carp reovirus(GCRV)is a disaster agent to aquatic animals,which belongs to genus
Aquareovirus.family Reoviridae.Sequences an alysis revealed GCRV S2 was 3877 nucleotides long
encoding a 1 38kDa protein VP2,which is deduced as virus RNA polymerase.To understan d the ..., Abstract
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In order to compare the difierence of protein expression in A utograph cdifornica nu,cleo,7
polyhedrosis virus(AcMNPV)mutants,the spodopterafrugiperda IPLB-SF-21 Cell was infected with
wild type(HR3)and mutants(ts317,ts538,ts8),and incubated at permissive and nonpermissive ..., Abstract
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Abstract:Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively
studied at the first time.It showed that the stored GCRV873,GCRVs75 and GCRv876 at minus 3012 for
ten years still remained infectious to CIK cells,and the 3 virus titers could reach above 10 ..., Abstract
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The immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric ..., Abstract
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ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation ..., Abstract
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specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both  ̄(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes ..., Abstract
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The immunogenic comparison of three grass carp hemorrhage viruses, GCHV873, GCHV875 and GCHV876 derived from different areas has been carried out on the basis of the levels of the elicited serum antibodies in rabbits. The anti-GCHV873 antibody titre was 2 folds higher than GCHV875 and 8 folds than ..., Abstract
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Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached ..., Abstract
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