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Author [FENG Yan] article list of Virologica Sinica
The gene sequences of the Hemagglutinin and Nucleoprotein were downloaded from GenBank. Bioedit7.0 softwere was used to pairwise these sequences and to construct a phylogenetic tree to analyze the variation rate of measles virus strains. The results demonstrated that variation rate of hemagglutinin ..., Abstract
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A real-time reverse transcription-polymerase chain reaction (RRT-PCR) assay based on TaqMan-MGB probe was developed to rapidly detect avain influenza virus subtype H5. The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of ..., Abstract
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In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) ..., Abstract
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A real-time TaqMan-based RT-PCR assay was developed to rapidly detect the Severe acute respiratory syndrome-associate coronavirus(SARS-CoV). Primers and probes specific to the conserved region of the SARS-CoV genome were selected,and the reactive system and conditions were optimized to improve the ..., Abstract
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