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Author [KONG Xian-gang] article list of Virologica Sinica
An 864bp fragment at the 3’- end of the vp3 gene of Goose parvovirus (GPV) HG5/82 isolate was cloned by RT-PCR, and was cloned into pMD18-T Simple vector. Positive clones were identified by REN digestion and PCR and a recombinant plasmid was digested with BamHⅠand Hind Ⅲ and subcloned into the ..., Abstract
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Nucleocapsid (N) protein genes of 13 Infectious bronchitis virus (IBV) strains isolated from 9 provinces in China between 2000 and 2004 were sequenced and compared to 42 IBV reference strains. The results showed that all of the N genes of the 13 isolates were composed of 1230 nucleotides, but point ..., Abstract
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Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the Small Envelope (E) gene of avian Infectious bronchitis virus (IBV) LX4 strain and the gene was cloned into the pMD18-T vector. By digestion with restriction enzymes SalⅠand BamHⅠ, the E gene was subcloned into ..., Abstract
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The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised ..., Abstract
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The gene of nonstructural protein 9 of SARS-coronavirus(SARS-CoV) was amplified using PCR from the product derivated from reverse transcription of SARS-CoV genome RNA,and was inserted into the multiple cloning sites of the expression vector pGEX-6p-1.Recombinant strain induced with IPTG expressed ..., Abstract
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Non-structural and structural genes of Goose parvovirus (GPV) HG5/82 strain were amplified using polymerase chain reaction (PCR) method and cloned into the pMD18-T vector, respectively. The two fragments were sequenced and analyzed with reference GPV strains and Muscovy duck parvovirus(MDPV) ..., Abstract
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