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Author [LI Min-hong] article list of Virologica Sinica
In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) ..., Abstract
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The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by ..., Abstract
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In order to elucidate the relationship between genetic characteristics and measles outbreak,and the influence of antigenic variation on measles vaccine effect,we investigated the genetic characteristics and antigenic variation of measles viruses isolated in Zhejiang province during 1999~ 2003.Rats ..., Abstract
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A real-time TaqMan-based RT-PCR assay was developed to rapidly detect the Severe acute respiratory syndrome-associate coronavirus(SARS-CoV). Primers and probes specific to the conserved region of the SARS-CoV genome were selected,and the reactive system and conditions were optimized to improve the ..., Abstract
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Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical ..., Abstract
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No side effects were found locally and generally in 25 volunteers when the secordary immunization(one injecton) with inactivated EHF vaccine was done at a year or a year and half after primary immunization, The EHF virus antibodies were detected by PRNT in these volunteers who were negative of ..., Abstract
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