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Author [MAO Hai-yan] article list of Virologica Sinica
A real-time reverse transcription-polymerase chain reaction (RRT-PCR) assay based on TaqMan-MGB probe was developed to rapidly detect avain influenza virus subtype H5. The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of ..., Abstract
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In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) ..., Abstract
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The effects of the M gene on the molecular epidemiological characteristics of the Hantavirus ZT10 strain isolated from M. arvalis was investigated. The gene was amplified by RT-PCR and yielded the expected product of 3651 bp. The amplified fragment was then cloned into the T vector and confirmed by ..., Abstract
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Respiratory syncytial virus(RSV) was isolated from nasopharyngeal aspirates(NPA) collected of children suffering acute lower respiratory tract infection in the Children’s hospital of Zhejiang province in the winter of 2004. The isolated strains were identified by RT-PCR. The whole region of the G ..., Abstract
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In order to elucidate the relationship between genetic characteristics and measles outbreak,and the influence of antigenic variation on measles vaccine effect,we investigated the genetic characteristics and antigenic variation of measles viruses isolated in Zhejiang province during 1999~ 2003.Rats ..., Abstract
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Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical ..., Abstract
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