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Author [WANG Hua-lin] article list of Virologica Sinica
Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency and necessity to develop high-quality influenza vaccines in a safer, more efficient and ..., Abstract
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Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV ..., Abstract
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The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments ..., Abstract
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p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial ..., Abstract
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The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes ..., Abstract
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Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity ..., Abstract
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There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using ..., Abstract
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In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading ..., Abstract
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