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Author [WANG Rui-rui] article list of Virologica Sinica
HIV-protease coding sequence was amplified from HIV-1IIIB RNA by one- step RT-PCR. The PCR product was inserted into the pet28a expression vector with an additional ATG start codon before and TAA stop codon after the coding sequence. Positive clone was selected and transformed into E. coli BL21 ..., Abstract
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