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Author [Wen Li] article list of Virologica Sinica
The hepatitis A virus (HAV) is a positive-sense, single-stranded RNA virus of the genus Hepatovirus in the family Picornaviridae. HAV is a common agent causing acute liver disease worldwide and primarily transmitted by the fecal-oral route. Approximately 120 species of bats exist in ..., Abstract
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Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 ..., Abstract
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To create the pathogenicity model of severe acute respiratory syndrome (SARS) on macaca rhesus, the macacu rhesus was infected with SARS coronavirus (CoV) was detected in different samples coming from macaca rhesue in different days by virus isolation, immunoflurescence assay, pathological ..., Abstract
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To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 ..., Abstract
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To amplify M fragment from unknown Phleboviruses,members of 7 serocomplexes and 8 no
complex assigned Phleboviruses,total 42,were chosen and tested.Tl1e M segment amino acid sequences
Of 4 Phleboviruses in GenBank were aligned.Conserved regions were selected to design primers. ..., Abstract
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For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein,the primers
were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9.After the RT—PCR
the amplified cDNA was cloned into the pMD18一T. NS5 protein gene of DpCPV was found to be 963 ..., Abstract
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Suckling mice were inoculated with L6565 blLV for researehlng the pathogeny of L6565 moL!se
Leukemia Afterinoeulatica~,叽emol3y,eⅧ sacrificed r3r week.Theperipheral blood,thymus,[ymp畸node,
spleen and liver weTe removed from l’lflOIl~.|I11e tissues were used to observe tIl0 ..., Abstract
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The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay,reverse transcription polymerase chain reaction and cy—
tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec—
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The pathogenicity of herpes simplex virus(HSV)in mice宙as observed.The mice appears
syndrome and sign afterinoculation 4 days HSV could beisolatedinthe blood on 2hours po$tin—
fection.The isolation rate of HSV and levd of viremia were increased on 48 hours postirfection.
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A method of reverse - transcription polymerase chain reaction(RT - PCR) was established for detection of respiratory syncytical virus (RSV) RNA genome, The primers were designed based on the nucleotide sequence of RSV N gene. The results showed that the primers can't amplify the genomes of ..., Abstract
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Antiviral effect of Alternanthera philoxeroides Griseb(APG)was studied in oral for epi-demic hemorrhagic fever virus(EHFV)infection in suckling mice.The APG dosages of A,BandC groups were 5mg/(g·d),7.5mg/(g·d)and 10mg/(g·d),respectively. The results showedthat the survival rate of A,B and C group ..., Abstract
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Epidemic hemorrhagic fever virus origined from patients with epidemic hemorrhagic fever was inoculated into suckling mice,The results showed that the suckling mice died regularly,The viral antigenand pathological damage appeared in the brain,lung,liver and kidney,The characterization of ..., Abstract
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