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Author [YANG Shou-jing] article list of Virologica Sinica
To examine whether the recombinant protein Hsp70-NP could induce the N-specific CTLs, we immunized C57/BL6 mice with purified protein Hsp70-NP, NP or Hsp70. The lymphoproliferative responses and CTL activities of spleen cells from vaccinated mice were determined. Accordingly, in order to get the ..., Abstract
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Recombinant fusion prokaryotic expression vector pGEX-4T-1/hsp70-S was constructed by cloning genes Hsp70 and Hantaan virus S gene coding region into pGEX-4T and was expressed in E. coli. The Hsp70-NP fusion protein was separated and purified with GSTrapFF purification system. We ..., Abstract
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Abstract:To study the apoptosis of malignant carcinoma cells induced by Hantaan virus.SP2/0 cells
cultured in vitro were infected with Hantaan virus.Their apoptosis profiles were detected using
Giem sa staining,flow cytometry and immunohistochemistry staining.Giemsa staining showed ..., Abstract
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In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results ..., Abstract
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In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results ..., Abstract
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With in situ hybridization technique the localization and distribution of HSP70 mRNA and Hantaan virus RNA in the tissues of 30 cadavers with epidemic hemorrhagic fever (EHF), which were collected from different EHF epidemic foci in China, were examined. HSP70 mRNA was detected in most of the ..., Abstract
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emorrhagic fever with renal syndrome(HFRS)virus RNA and antigens were simultane-ously detected by in situ hybridization and immunohistochemical techniques in the autopsy renaltissues of the patients with HFRS.In 36 cases collected separately from Shaanxi,Shenyang,Shanghai and Jiangxi HFRS endemic ..., Abstract
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Immunocytochemistry and in situ hybridization were performed on the autopsy tissues of 39 fetal cases of patients with hemorrhagic fever with renal syndrome to search for viral in-clusion body(IB)and study its antigenic and nucleic acid characters.The tissues with IB were further observed by ..., Abstract
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In situ hybridization was used for detection of hantavirus RNA in the tissues of 1 9 cadavers with hemorrhagic fever w ith renal syndrome (HFRS) collected from different HFRS endemic foei in China. Viral RNA wiLq detec ted in a11 the 1 9 cases. It was mainly located in epithelial or ..., Abstract
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The viral antigens in 16 kinds of 10% formalin-fixed paraffin-embedded tissues of 19 fatal cases due to the death of hemorrhagic fever with renal syndrome (HFRS) were detected and anslyzed by 15 clones of monoclonal antibodies (MAbs) respectively against glycoprotein Ⅱ(G2), nucleocapsid (NP) and ..., Abstract
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Using polyclonal antibody (PAb)and monoclonal antibodies(MAbs) against epidemic hemorrhagic fever virus (EHFV), the viral antigens in fresh and long-term storage EHF autopsy tissues were studied comparatively with more sensitive multi-layer PAP and PAP combined with ABC (PAP+ABC) techniques. The ..., Abstract
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In the detecton of epidemic hemmorrhagic fever viral antigen in the autopsy and biopsy tissues by immunocytochemical methods, it was found that the monoclonal antibodies to nuclear antigen could stain antigen well with a rather diffuse pattern in fresh and well fixed material only, Polyclonal ..., Abstract
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