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Author [YANG Song-tao] article list of Virologica Sinica
Membrane (M) protein genes of Canine coronavirus (CCoV) domestic strain DXMV, V1 and V2 were successfully amplified, sequenced and analyzed. The full length of all the M gene of DXMV, V1 and V2 strains was 792 bp, encoding 263 amino acids. The initial 17 amino acids as appeared to be a signal ..., Abstract
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The nucleocapsid protein gene (N) located 108 to 1679 situation of Canine distemper virus (CDV) genome and was immunogenicity protein with strong conservatism, so N gene was choosed as aim gene. A shuttle plasmid pVAXΔE3LPN containing the gene encoding the nucleocapsid protein of CDV was ..., Abstract
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The rationale of this study was to elucidate the etiological agent characteristics and pathogenic mechanisms of the Avian H5N1 influenza virus in mammals, and to offer a platform for evaluating vaccines and drugs against AIV. Male BALB/c mice (18~20g) were anesthetized by inhalation of ether and ..., Abstract
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The intercellular adhension molecular-1(ICAM-1)and it’s its receptors in the mice’s lungs of HPAIV and control mice pneumonia were detected by using immunohistochemistry staining after infected mice with Highly pathogenic avian influenza virus (HPAIV). The expressive level of ..., Abstract
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In this study, the major antigenic region of spike (S) gene of Canine coronavirus giant panda’s isolate (CCV strain DXMV) was expressed in Pichia pastoris. The S1 gene fragment encoding major antigenic region of S protein was amplified and cloned into T vector, then subcloned into pPICZαA for ..., Abstract
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Spike (S) gene of Canine coronavirus giant panda’s isolate (CCV strain DXMV) was amplified and cloned into pGEM-T for sequencing. The complete length of spike gene of strain DXMV was 4362 bp, which encoded 1453 amino acids. The initiative 18 amino acids were signal peptide. The homologies of ..., Abstract
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