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Author [Ye Li] article list of Virologica Sinica
NAP1L1 has been shown to function in the life cycle of several DNA virus and retrovirus. However, whether NAP1L1 regulates hepatitis C virus (HCV), a positive-stranded RNA virus, remain to be elucidated. In this study, we identified NAP1L1 as a novel binding partner for HCV NS3. Our results suggest ..., Abstract
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An epidemic of Chinese ferret badger-associated human rabies was investigated in Wuyuan county, Jiangxi province and rabies viruses isolates from ferret badgers in different districts in Jiangxi and Zhejiang provinces were sequenced with their nucleotides and amino acids and aligned for ..., Abstract
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HBV X gene was amplified by PCR and cloned into the prokaryotic expressing vector pET-his and the eukaryotic expressing vector pcDNA3.1(-). E.coli BL21 (DE3) were transformed by the recombinant plasmid pET-his-HBx.. HBx protein was expressed by IPTG induction and formed insoluble inclusion bodies ..., Abstract
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A recombinant HCV RNA-dependent RNA polymerase (RdRp, NS5B protein) was expressed in E. coli strain BL21(DE3). The purified protein was used to investigate the RNA syntheses from the 3′ terminal sequences of HCV positive and negative strand RNA in vitro. The positive strand RNA did not direct the ..., Abstract
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Coding sequences of Transfusion transmiffed virus(TT virus or TTV) ORF2 was amplified by PCR, using plasmid pET-His-TTV2 containing the full-length ORF2 of TTV as template. The amplified fragment of 606bp was cloned into plasmid pEGFP-N1 and GFP-VP2 fusion protein was expressed. The recombinant ..., Abstract
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Full-length NS5A gene of Hepatitis C virus(HCV) was amplified by PCR, using the plasmid pBRTM/HCV 1-3011 containing HCV full-length open reading frame(ORF)as template, and cloned into the eukaryotic expressing plasmid pcDNA3.1(-)by DNA recombination technique. The recombin- ant vector was ..., Abstract
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Recombinant plasmid pCNS2 was constructed by inserting Hepatitis C Virus full-length NS2 gene between Xho I and Hind III sites of an eukaryotic expression vector pCDNA3.1(-). The recombin- ant plasmid pCNS2 was transfected to Huh-7 cells with LipoVecTM and HCV NS2 mRNA and protein produced in ..., Abstract
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Abstract:Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1
core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3.The
recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec .Expression of ..., Abstract
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