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Author [Yuan Li] article list of Virologica Sinica
Characteristics of HIV-1 Molecular Epidemiology in Suzhou, China, from 2009 to 2014
Ying Yuan, Shengjie Tang, Yuan Li, Dongmei Yan, Qunxin Peng, Xuerong Ya, Yanhui Song
Suzhou is experiencing a dynamic human immunodeficiency virus (HIV)/acquired immune deficiency syndrome (AIDS) epidemic. While serology based surveillance systems have reported the spread of HIV/AIDS, detailed tracking of its molecular epidemiology and transmission in populations remains mostly ..., Abstract
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2018 Vol. 33 (2): 209
Inhibitory Effects of Ginsenoside Rb1 on Apoptosis Caused by HSV-1 in Human Glioma Cells
Yuan-Yuan Liang, Bin Wang, Dong-Meng Qian, Ling Li, Zhi-Hao Wang, Ming Hu, Xu-Xia Song
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were ..., Abstract
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2012 Vol. 27 (1): 19-25
Ion Channels as Antivirus Targets
Xin LIANG, Zhi-yuan LI
Ion channels are membrane proteins that are found in a number of viruses and which are of crucial physiological importance in the viral life cycle. They have one common feature in that their action mode involves a change of electrochemical or proton gradient across the bilayer lipid membrane which ..., Abstract
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2010 Vol. 25 (4): 267-280
Construction of a Phage Display Random Decapeptide Library and Panning for Mimic Antigen Epitope of ScFv A1 Against WSSV
YUAN Li, WANG Yu-zhen, XIAO Nan, ZHANG Xiao-hua, DAI He-ping
A phage display random decapeptide library with high titer was constructed using pCANTAB 5 E as vector and used to select and identify antigen epitopes. ScFv A1, which specifically binds the shrimp’s White spot syndrome virus (WSSV), was used to pan against the decapeptide library and a random ..., Abstract
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2006 Vol. 21 (6): 614-618
Deletion of p10 From the Genome of HaSNPV Results in a Faulty Polyhedral Envelope
DONG Chun-sheng, DENG Fei, YUAN Li, PAN Xiao-yu, WANG Hua-lin, HU Zhi-hong
p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial ..., Abstract
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2006 Vol. 21 (6): 594-598
The Transcription Profiles of Nine HaSNPV Genes
DAI Wen-tao, DENG Fei, WANG Hua-lin, YUAN Li, HU Zhi-hong
The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes ..., Abstract
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2006 Vol. 21 (1): 47-51
Construction of a Recombinant HaSNPV Expressing GP64
WANG Shu, WANG Hua-lin, DENG Fei, WU Dong, LONG Gang, YUAN Li, HU Zhi-hong
There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using ..., Abstract
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2005 Vol. 20 (1): 79-83
Molecular Cloning and Expression of the fp25k Gene of HaSNPV in E. coli and Preparation of Its Antiserum
WU Dong, DENG Fei, HU Zhi-hong, YUAN Li, SUN Xiu-lian
The PCR product of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus HaSNPV fp25k gene was cloned into a prokaryotic expression vector pProEXHTb. After inducing with IPTG, the fp25k gene was successfully expressed in E.coli DH5α. The expression product was 32 kDa. This protein was ..., Abstract
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2004 Vol. 19 (4): 380-384
Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo
LU Yu-rong, FENG Zong-di, WU Dong, SUN Xiu-lian, DENG Fei, YUAN Li, J.M.Vlak, HU Zhi-hong
The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The ..., Abstract
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2004 Vol. 19 (3): 276-280
Recent Advances on Envelope Fusion Proteins of Baculoviral Budded Viruses
WANG Shu, YUAN Li, HU Zhi-hong
Abstract
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2004 Vol. 19 (1): 82-86
Pathogenic and Immunohistochemical Studies of the HaSNPV Infected Helicoverpa armigera Larvae
OUYANG Zhi-quart, SUN Xiu-lian, DENG Fei, YUAN Li, HU Zhihong
Abstract：Here we report the pathogensis process of Helicoverpa armigera larvae infected with
Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)using H．E staining and
immunohistoc hemistry with an tibody against polyhedrin．The fourth instar Helicoverpa armigera larvae ..., Abstract
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2003 Vol. 18 (6): 603-606
Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies
CHEN M ing—ruing, WU Dong, YUAN Li, CHEN Xin—wen, HU Zhi—hong
Abstract：Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the
genome DNA of HaSNPV．The PCR products were cloned into pBluescript KS(+)plasmid．The genes
were introd uced into the expression vector pProExHTb．After IFFG induction．the E coli DH5a strain ..., Abstract
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2003 Vol. 18 (6): 597-602
Detection of Human Cytomegalovirus Antigen in Urine Samples by Enzyme-linked Immunosorbent Assay
ZHANG Xin, YUAN Li, ZHI Zai-Xin, CHEN Xiu-Zhu, WU Yuan-Ming
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of human cytomegalovirus (HCMV) in urine samples There were 4 kinds of method of the ELISA and monoclonal antibody directed against 20 or 130 kilodalton envelope structural polypeptides of HCMV was served as a capture ..., Abstract
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1991 Vol. 06 (2): 102
ISOLATION AND MORPHOLOGICAL OBSERVATION OF PARASA CONSOCIA GRANULOSIS VIRUS
DENG Hai-Fan, YUAN Li, ZHANG Shi-Min, BANG Ke-Cun
Abstract
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1987 Vol. 02 (3): 107
BRIGHT AND DARK FIELD ELECTRON MICROSCOPY OF NUCLEAR ACID MOLECULES OF SOME VIRUS
YUAN Li, WANG Xue-Lan, DENG Hai-Fan
Abstract
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1986 Vol. 01 (2): 58
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