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Author [ZHANG Zhong-Xin] article list of Virologica Sinica
In this paper, the function of the ie1 gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), belonging to group II nucleopolyhedrovirus, was studied in mammalian cells. We amplified the SeMNPV ie1 gene and expressed it by fusing to the C terminal of enhanced GFP protein in ..., Abstract
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Primers based on published sequences of granulin genes were synthesized and used to amplify the granulin gene from Clostera anachoreta granulovirus (ClanGV). The generated product was se-quenced. Sequence analysis indicated the entire ORF of ClanGV granulin gene was 747bp, encoding a protein of 248 ..., Abstract
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The activity of two isolates of Spotoptera exigua nucleopolyhedrovirus , SeMNPV-M and SeMNPV-Z, was compared by bioassays. The LD50 values of SeMNPV-M and SeMNPV-Z in 3rd instar host larvae were 195. 8 PIBs/ gram diet and 242. 4 PIBs/gram diet, respectively. The LT50 values infected with ..., Abstract
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A putative v-cathepsin(v-cath) gene of Spodoptera exigua nucleopolyhedrovirus(SeMNPV) was cloned,sequenced and expressed.The results showed that the gene size was 1014 nucleotides,encoding a 338 amino acids putative protein.The amino acid sequence alignment of ten baculoviral v-CATHs showed that ..., Abstract
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By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus(SeNPV)has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin ..., Abstract
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A ubiquitin gene of Spotoptera exigue multi-nucleopolyhedrovirus (SeMNPV) was
cloned and sequenced.The result showed that the gene size was 243 bp,encoded 80 amino acids with
apredicted size of 9.4kDa.To construct expressive vector for the udiquitin gene of SeMNPV.the frag.
ment ..., Abstract
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Seven distinct genotypes were isolated from wild type Helicoverpa armigera single nucleocapsid NPV (HaSNPV W)by low mortality dose infection of 3rd instar H.armigera and progency viral DNAs extracted from individual cadaver were analysed by endonucleases.Each of the 7 genotypes had different EcoRI ..., Abstract
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It is the first time in china that the study of an insect virus gains commercial successes.This is owlng to the development of a new formulation──emulsifiable suspension, the im-provement of tectinology and facilities,which resulted in raising production efficiency and re-ducing cost. In this ..., Abstract
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Bioassay of a Nuclear Polyhedrosis Virus propagated either in vivo or vitro was conducted on third-instar Buzura suppressaria larvae which were reared on an artificial diet contaminated with virus, The regression equation between dosage and mortality were:Y=3.1815+0.697XY=3.2000+0.665XThe LD_(50) ..., Abstract
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