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Articles list with the same subject [Characterization] of Virologica Sinica
The Eurasian avian-like swine influenza A (H1N1) viruses (EAS-H1N1) have circulated in pigs since 1979, and human sporadically infected with EAS-H1N1 have been reported in several countries. In October 2016, the EAS-H1N1 infection in an adult hospitalized patient with severe pneumonia was ..., Abstract
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The chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, which has infected millions of people in Africa, Asia, Americas, and Europe since it reemerged in India and Indian Ocean regions in 2005–2006. Starting in the middle of November 2016, CHIKV has been widely spread, and more than ..., Abstract
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Here, we describe a cell culture-based procedure for isolating the infectious ZIKV (GenBank KU963796) from a human serum sample (ca. 50 μL) with an extremely low viral load (Ct value = 32)., Abstract
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As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a “living fossil” for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and ..., Abstract
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Cyanophages are ubiquitous and essential components of the aquatic environment and play an important role in the termination of algal blooms. As such,they have attracted widespread interest. PP was the first isolated cyanophage in China,which infects Plectonema boryanum and Phormidium foveolarum. ..., Abstract
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Currently, three predominant subtypes of influenza virus are prevalent in pig populations worldwide: H1N1, H3N2, and H1N2. European avian-like H1N1 viruses, which were initially detected in European pig populations in 1979, have been circulating in pigs in eastern China since 2007. In this study ..., Abstract
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The epidemic of rabies has rapidly increased and expanded in Yunnan province in recent years. In order to further analyze and understand the etiological reasons for the rapid expansion of rabies in Yunnan, a strain of rabies virus CYN1009D in Yunnan was isolated, and the complete genomic sequencing ..., Abstract
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Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein ..., Abstract
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The polymerase gene, a non-structural gene from strain TS of transmissible gastroenteritis virus (TGEV), was amplified by RT-PCR primers designed based on the Purdue nucleotide sequence in GenBank. The expected 20054 bp product was obtained. The nucleotide sequence of ORF1 of TS shared nucleotide ..., Abstract
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The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised ..., Abstract
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Abstract:Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively
studied at the first time.It showed that the stored GCRV873,GCRVs75 and GCRv876 at minus 3012 for
ten years still remained infectious to CIK cells,and the 3 virus titers could reach above 10 ..., Abstract
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Some characterizations of four Grass Carp Reovirus (GCRV) strains were comparatively studied at the first time. It showed that the stored GCRV\-\{873\},GCRV\-\{875\} and GCRV\-\{876\} at minus 30℃ for ten years still remained infectious to CIK cells, and the 3 virus titers could reach above 10\+2 ..., Abstract
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Twenty three serum samples from blood donor, hemodialysis and hepatitis patients were collected. Using reverse transcription nested polymerase chain reaction method (RT nested PCR),the cDNA fragments of the putative envelope protein 2 (E 2/NS 1) of HCV RNA were amplified and then sequenced. ..., Abstract
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