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Articles list with the same subject [Core] of Virologica Sinica
Hepatitis B virus (HBV) infection is one of the major problems that threatens global health. There have been many studies on HBV, but the relationship between HBV and host factors is largely unexplored and more studies are needed to clarify these interactions. Filamin B is an actin-binding ..., Abstract
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Similar to Hepatitis C virus (HCV) infection in humans, HCVcc infection can also result in persistent and chronic infection. The core protein is a variable protein and exists in several sizes. Some sizes of core proteins have been reported to be related to chronic HCV infection. To study the ..., Abstract
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Emerging data indicated that HCV subverts the antiviral activity of interferon (IFN); however, whether HCV core protein contributes to the process remains controversial. In the present study, we examined the effect of HCV core protein on interferon-induced antiviral gene expression and whether the ..., Abstract
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PCR method was used to Construct multi-epitope gene ss1c containing HBV S(1-222AA),preS1(10-50AA)and C(1-30AA) gene.The fusion ss1c gene was cloned into the expression vector pPIC3.5k.The recombinant vector was transformed into the host cell of GS115 with electroporation method.After screening with ..., Abstract
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Abstract:In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1
(HIV-I)core protein gene(pCI—neoGAG),gag gene was acquired from the plasmid pKSGAG by
restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector ..., Abstract
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Abstract:Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1
core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3.The
recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec .Expression of ..., Abstract
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In order to construct bicistronic expression vector of RANTES and SDF-l,the ligands of HIV-1 principal coreceptors,RANTES-KDEL were amplified from pCMV-R-K by PCR and ligased with eukaryotic expression vector pCMV-S/K. The construction of pCMV-R-K-S-K was confirmed by enzymatic digestion and ..., Abstract
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In order to understand the mechanisms of hepatitis B virus (HBV) in the development of hepatocellular carcinoma, the nested PCR was used to amplify HBV core promoter from tumour tissues of 14 HBV DNA positive patients with hepatocellular carcinoma, and 10 serum samples of hepatitis B patients and ..., Abstract
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The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core+NS3) and pHCV NC (NS3+Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was ..., Abstract
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The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the ..., Abstract
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