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Articles list with the same subject [Monoclonal antibody] of Virologica Sinica
Wheat dwarf disease caused by wheat dwarf virus (WDV) is currently present in wheat growing regions in China and causes serious losses in wheat yield. To develop reliable and effective serological detection methods for WDV, the coat protein (CP) gene of WDV was cloned and expressed in ..., Abstract
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The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor (F0) and then cleaved into a disulfide-linked F1 and F2 subunits during the ..., Abstract
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Citrus tristeza virus (CTV) is one of the most economically important citrus viruses and harms the citrus industry worldwide. To develop reliable and effective serological detection assays of CTV, the major capsid protein (CP) gene of CTV was expressed in Escherichia coli BL21 (DE3) using ..., Abstract
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Prion diseases are a group of neurodegenerative diseases that are fatal. The study of these unique diseases in China is hampered by a lack of resources. Amongst the most important resources for biological study are monoclonal antibodies. Here, we characterize a panel of monoclonal antibodies ..., Abstract
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P74 is a per os infectivity factor of baculovirus. Here, we report the production of three monoclonal antibodies (mAbs), denoted as 20D9, 20F9 and 21E1, raised against P74 of Helicoverpa armigera nucleopolyhedrovirus (HearNPV), and the identification of their recognition epitopes. The full-length ..., Abstract
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To identify linear epitopes on the non-structural protein 3AB of foot-and-mouth disease virus (FMDV), BABL/c mice were immunized with the 3AB protein and splenocytes of BALB/c mice were fused with myeloma Sp2/0 cells. Two hybridoma monoclonal antibodies (mAbs) cell lines against the 3AB protein of ..., Abstract
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In order to develop an anti-FMDV A Type monoclonal antibody (mAb), BABL/c mice were immunized with FMDV A type. Monoclonal antibodies (mAbs) 7B11 and 8H4 against Foot-and-mouth disease virus (FMDV) serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with ..., Abstract
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In this study, a panel of monoclonal antibodies (mAbs) against Porcine reproductive and respiratory syndrome virus(PRRSV), named as 8C9 and4B4, were produced by fusing SP2/0 myeloma cells and spleen cells of BALB/c mice immunized with the PRRSV (TCID50=5.5), screened by the indirect ELISA and ..., Abstract
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The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV) was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21. After induction, the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal ..., Abstract
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In the present study, a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres ..., Abstract
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A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and ..., Abstract
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Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. ..., Abstract
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In order to develop an anti-FMDV Asia1 type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5E1 and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asia1 mAbs had no cross-reactivity with Swine vesicular ..., Abstract
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In order to characterize the specificity of the monoclonal antibodies (McAbs) against SARS- associated coronavirus (SARS-CoV) nucleocapsid protein and to identify the epitopes recognized by the McAbs,the nucleocapsid proteins of human coronavirus OC43 (HCoV-OC43) and 229E (HCoV-229E) was expressed ..., Abstract
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Structural and envelope glycoprotein E2 (gp55) of Classical swine fever virus (CSFV) is the most antigenic protein being responsible for eliciting neutralizing antibodies and conferring protective immunity. Infection of cells with CSFV is mediated by the interaction of E2 and Erns with the cell ..., Abstract
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Monoclonal antibody of F 3 hybridoma cell strain against RSV specific antigen was prepared by hybridoma and tested by indirect immunofluorescence and ELISA. It has neutralizing and fusion inhibiting activity. The neutralizing titres and fusion inhibiting titres of ascites of mice are 1∶128 and ..., Abstract
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An indirect ELISA by using monoclonal antibody (McAb) against hutnan IgM (μ chain)as the antibody labeled with horseradish peroxidase (second antibody) is used in detecion Rubela virus - specific IgM for early diagnosis of Rubella virus infection. Through the detection and verifition of a large ..., Abstract
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This study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal ..., Abstract
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The viral antigens in 16 kinds of 10% formalin-fixed paraffin-embedded tissues of 19 fatal cases due to the death of hemorrhagic fever with renal syndrome (HFRS) were detected and anslyzed by 15 clones of monoclonal antibodies (MAbs) respectively against glycoprotein Ⅱ(G2), nucleocapsid (NP) and ..., Abstract
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Using hybridoma techniques, six monoclonal antibodies (mAb), P3, 4A4 6B1, 7A6, 7D1 and 7D8 to Freesia chlorotic stripe mosaic virus ( FCSMV), were prepared. The titers of mAb were 1:10~2—1:10~6. MAb 7A6 and 7D1 were IgG1 subclass. 7D8 and 4A4 were IgG2a and lgG2b subclass, respectively, mAb P3 and ..., Abstract
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