Articles list with the same subject [NS1] -- Virologica Sinica

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Articles list with the same subject [NS1] of Virologica Sinica

Progress towards understanding the pathogenesis of dengue hemorrhagic fever

Xiaojing Pang, Rudian Zhang, Gong Cheng

Dengue virus (DENV) is a mosquito-borne virus belonging to the Flaviviridae family. There are 4 serotypes of DENV that cause human disease through transmission by mosquito vectors. DENV infection results in a broad spectrum of clinical symptoms, ranging from mild fever to dengue hemorrhagic ..., Abstract

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2017 Vol. 32 (1): 16

Critical role of Dengue Virus NS1 protein in viral replication

Jingjing Fan, Yi Liu, Zhiming Yuan

Dengue virus (DENV) nonstructural protein 1 (NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites (Asn-130 and Asn-207) and 12 conserved cysteine (Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results ..., Abstract

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2014 Vol. 29 (3): 162-169

A Pair of Novel Primers for Universal Detection of the NS1 Gene from Various Bluetongue Virus Serotypes

Hui-qiong YIN, Gai-ping ZHANG, Hong ZHANG, Jin-gang ZHANG

Twenty five serotypes of Bluetongue virus (BTV) have been identified worldwide. Rapid and reliable methods of virus universal detection are essential for fighting against bluetongue (BT). We have therefore developed and evaluated a pair of primers which can detect various serotypes of BTV by ..., Abstract

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2008 Vol. 23 (1): 68-72

Cloning and Construction of Prokaryotic Expression Vector of ns1 Gene of Influenza A Virus

ZHANG Zhi-zhen, HONG Wen-shan, LI Kang-sheng

To understand the characteristics of the ns1 gene of Avian influenza A virus H5N1 and to prepare NS1 protein in scale, viruses were cultivated in embryonated hen eggs and virion RNA was extracted from allantoic fluid. The complete ns gene was amplified by RT-PCR and analyzed. Sequence analysis ..., Abstract

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2004 Vol. 19 (5): 449-453

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

LIU Yan-hua, FAN Wei-xing, WANG Xi-le, LIU Wen-qiang, XU Chuan-tian, ZHANG Zhi

NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5% ..., Abstract

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2004 Vol. 19 (4): 353-355

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